Alkaline Phosphatase Staining Kit II 堿性磷酸酶染色試劑盒

【溫馨提示I】：見(jiàn)堿性磷酸酶産品專題，了解更多(duō)相(xiàng)關産品資料；

【溫馨提示II】：見(jiàn)堿性磷酸酶活性檢測試劑盒産品；

【溫馨提示III】：見(jiàn)堿性磷酸酶常用生(shēng)化底物(wù)及試劑盒産品；

基本介紹（Stemgent，00-0055）：

處于未分化狀态的胚胎幹細胞（ES）和誘導多(duō)潛能(néng)幹細胞（iPS）典型特征為(wèi)具高(gāo)水(shuǐ)平的堿性磷酸酶表達，同時結合其他的一(yī)些表面标志(zhì)物(wù)，來顯示未分化細胞具自(zì)我更新的潛能(néng)。堿性磷酸酶（ALP，或AP）是一(yī)種水(shuǐ)解酶，堿性條件(jiàn)下(xià)能(néng)夠将核苷酸、蛋白(bái)、甾醇等分子去磷酸化作用。

The undifferentiated state of embryonic stem (ES) and induced pluripotent stem (iPS) cells can be characterized by a high level of alkaline phosphatase (AP) expression which, along with the expression of other surface markers, indicates undifferentiated cells with the potential to self-renew.AP is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. When fixed ES or iPS cells are stained using the AP Staining Kit, undifferentiated cells appear red or purple, whereas differentiated cells appear colorless.

結果呈現：

固定ES或iPS用堿性磷酸酶染色試劑盒（Stemgent，00-0055）染色後，未分化細胞呈紅(hóng)色或紫色，而分化細胞無色。

産品特性：

1. 試劑盒組分

1. 2. 保存和穩定性

4°C保存，不可凍融。按照(zhào)指示保存收到(dào)貨後至少能(néng)存放(fàng)6個(gè)月(yuè)。

1. 3. 質量控制

本堿性磷酸酶染色試劑盒以人和小(xiǎo)鼠幹細胞為(wèi)對象進行功能(néng)測試，以确保産品質量。（The AP Staining Kit II is functionally tested on human and mouse ES cells to ensure product quality.）

訂購信息：Stemgent原裝進口，幹細胞研究專家精品，品質認證，歡迎選購。

00-0055 Datasheet

00-0055 Staining Protocol

相(xiàng)關信息：自(zì)産堿性磷酸酶染色試劑盒，在保證優秀質量的情況下(xià)，提供更有競争力的價格，歡迎選購。

參考文獻：

Pease, S., Braghetta, P., Gearing, D., Grail, D., and Williams, R.L. (1990) Isolation of embryonic stem (ES) cells in media supplemented with recombinant leukemia inhibitory factor (LIF). Dev Biol. 141: 344-352.

Smith, A.G., Nichols, J., Robertson, M., and Rathjen, P.D. (1992) Differentiation inhibiting activity (DIA/LIF) and mouse development. Dev. Biol. 151: 339-351.

Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872.

Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318: 1917-1920.

Additional Publications

Momcilovic O; Sivapatham R; Oron TR; Meyer M; Mooney S; Rao MA; Zeng X. "Derivation, characteriazation, and neural differentiation of integration-free induced pluripotent stem cell lines from Parkinson's disease patients cfarrying SNCA, LRRK2, PARK2, and GBA mutations." PLoS ONE 11(5): e0154890. doi:10.1371/journal.pone.0154890 (2016)

 — — Written/Edited by V. Shallan【版權歸MKBio懋康所有】

上海夢澤生物科技有限公司是一(yī)家涉足于生(shēng)命科學和生(shēng)物(wù)技(jì)術(shù)領域研究的試劑、儀器(qì)和實驗室消耗品與實驗服務工(gōng)作，主要從(cóng)事(shì)細胞生(shēng)物(wù)學、植物(wù)學、分子生(shēng)物(wù)學、免疫學、生(shēng)物(wù)化學、蛋白(bái)組學。生(shēng)物(wù)制藥與診斷試劑研發生(shēng)産等領域。 本公司秉承“以人為(wèi)本，以誠為(wèi)信、合同守信”的經營理念。堅持"品質保障"的原則為(wèi)廣大客戶提供優質産品。