【溫馨提示II】：見(jiàn)堿性磷酸酶染色試劑盒産品；

【溫馨提示III】：見(jiàn)堿性磷酸酶常用生(shēng)化底物(wù)及試劑盒産品；

産品關鍵詞：

Phosphatase, Alkaline堿性磷酸酶；ALP/AKP；對硝基苯酚磷酸鹽（pNPP）；偶氮偶聯法；顯色法（定量）；染色法（定性）；

基本介紹：

► 堿性磷酸酶（Phosphatase, Alkaline，ALP/AP/AKP/ALKP/ALPase，EC 3.1.3.1），是一(yī)種含兩個(gè)相(xiàng)同亞基的二聚體，也是一(yī)種約含12%碳水(shuǐ)化合物(wù)（6%己糖和6%其他中性糖）的糖蛋白(bái)，每個(gè)ALP分子含4個(gè)鋅離子和4個(gè)二硫鍵。鎂離子存在情況下(xià)，ALP可達到(dào)最高(gāo)酶活。堿性條件(jiàn)下(xià)，ALP能(néng)催化磷酸單酯水(shuǐ)解，比如對硝基苯酚磷酸鹽（pNPP）、磷酸苯酯、葡萄糖-6-磷酸、葡萄糖-1-磷酸、果糖-6-磷酸、腺苷-5-磷酸等。

► 常見(jiàn)的堿性磷酸酶ALP包括腸道堿性磷酸酶（如小(xiǎo)牛腸來源，calf intestinal）、胎盤堿性磷酸酶（placental alkaline phosphatase）、非組織特異性堿性磷酸酶（tissue-nonspecific isozyme, alkaline phosphatase）等。

► ALP常用來偶聯抗體和其他蛋白(bái)，從(cóng)而滿足ELISA、WB和組織化學檢測上(shàng)的應用。通(tōng)常用來蛋白(bái)的去磷酸化實驗，比如酪蛋白(bái)和核酸等。當需要高(gāo)檢測靈敏度時也能(néng)用來蛋白(bái)标記。還(hái)可用在DNA或RNA 的5′-末端去磷酸以防止自(zì)連。經堿性磷酸酶的去磷酸化作用後，DNA 或 RNA 還(hái)可被放(fàng)射性标記磷酸鹽标記（通(tōng)過 T4 多(duō)核苷酸激酶）。

研究意義：

堿性磷酸酶（ALP）活性的變化與一(yī)系列生(shēng)理和病理事(shì)件(jiàn)緊密關聯，比如骨骼發育、骨骼相(xiàng)關疾病、妊娠相(xiàng)關疾病和炎症性腸病（inflammatory bowel diseases）等。正因如此，ALP被用作衡量生(shēng)理特征或疾病的參照(zhào)指标。另外，ALP活性檢測在環境監測、食品質量控制等方面也呈現重要作用。

► 幹細胞如誘導多(duō)潛能(néng)幹細胞iPS，ALP活性很高(gāo)，則ALP常被用作iPS誘導成功的标志(zhì)；

► 分化的結腸癌細胞内ALP活性顯著提高(gāo)，則ALP常被當做結腸癌細胞分化程度的指标（定性/定量）；

► 血清中ALP水(shuǐ)平提高(gāo)，被稱作高(gāo)堿性磷酸酶血症，此症狀被認為(wèi)和惡性膽管阻塞、原發性膽管硬化、原發性硬化膽管炎、肝淋巴瘤和肝肉瘤等肝膽疾病密切相(xiàng)關；

► ALP是成骨細胞的副産物(wù)，血清中ALP活性升高(gāo)和骨頭發育密切關聯；

► 血清ALP活性過低(dī)也與一(yī)些疾病相(xiàng)關；

► 兒童和孕婦血清内ALP活性較常人高(gāo)一(yī)些；血清中ALP活性範圍在20-140U/L。

檢測原理（堿性磷酸酶活性）：

對硝基苯酚磷酸鹽（pNPP）是一(yī)種常用的堿性磷酸酶（ALP）顯色底物(wù)，堿性條件(jiàn)下(xià)能(néng)被ALP催化生(shēng)成對硝基酚（para-nitrophenol，p-nitrophenol），呈黃色産物(wù)，可在400-415nm檢測到(dào)吸光(guāng)度。産物(wù)黃色越深，代表ALP活性越高(gāo)，反之則酶活性越低(dī)。則通(tōng)過比色分析可定量檢測ALP水(shuǐ)平。

反應式如下(xià)：

檢測對象：

細胞或組織裂解液或勻漿液、血清、血漿、尿液等樣品内源性堿性磷酸酶活性。

訂購信息I：Anaspec國(guó)原裝進口，>幾十篇文獻引用，高(gāo)口碑産品。

試劑盒組分：

AS-72146 SensoLyte pNPP Kit Datasheet

訂購信息II：國(guó)産堿性磷酸酶檢測試劑盒，在保證優秀質量的情況下(xià)，提供更有競争力的價格，歡迎選購。

試劑盒組分：

引用文獻（Anaspec，AS-72146）：

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 — —Written/Edited by V. Shallan【版權歸MKBio懋康所有】