FITC-Con A (FITC labeled Concanavalin A)

FITC标記刀豆蛋白(bái)A

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産品标簽

淋巴細胞轉化實驗（T Lymphocyte Transformation Test），非特異性有絲分裂原（mitogen），T細胞活化和增殖，Con A，Concanavalin A ，伴刀豆凝集素，植物(wù)血球凝集素PHA，CAS：11028-71-0

産品信息

植物(wù)凝集素系列相(xiàng)關産品：

1）  見(jiàn)我司懋康生(shēng)物(wù)整理的植物(wù)血凝素PHA（PHA-L，PHA-M，PHA-E，PHA-P）産品專題和信息。

2）  見(jiàn)我司懋康生(shēng)物(wù)整理的番茄凝集素Tomato-Lectin産品專題和信息。

3）  見(jiàn)我司懋康生(shēng)物(wù)整理的加納籽凝集素I-同工(gōng)凝集素B4（GSL I-B4，BSL I-B4）産品專題和信息。

4）  見(jiàn)我司懋康生(shēng)物(wù)整理的刀豆蛋白(bái)凝集素Concanavalin A産品專題和信息。

5）  更多(duō)凝集素産品逐漸開(kāi)發中。。。。。。

産品描述

刀豆蛋白(bái)A（Concanavalin A，Con A），來源于Canavalia ensiformis洋刀豆（刀豆），是一(yī)種凝集素蛋白(bái)（Mw 104kDa）。pH≥7.0條件(jiàn)下(xià)，Con A以同質四聚體結構形式存在，由四個(gè)分子量為(wèi)26kDa的亞基構成。酸性條件(jiàn)（pH 4.5-5.5）下(xià)，Con A解離為(wèi)活化的二聚體結構（52kDa）。每一(yī)個(gè)單體，不管pH值或分子結構如何，都包含2個(gè)金屬離子結合位點。金屬離子（Ca2+ 和 Mn2+ ）必須結合到(dào)這兩個(gè)位點上(shàng)Con A才能(néng)發揮活性。乙酰化、琥珀酰化或其他衍生(shēng)物(wù)也能(néng)産生(shēng)穩定的二聚體結構形式。

刀豆蛋白(bái)A（Concanavalin A，Con A）無血型特異性，但選擇性結合發現于各種糖蛋白(bái)、糖脂和糖類分子上(shàng)的α-甘露糖基（α-mannosyl residues）和α-葡糖基殘基（α-glucosyl residues），需要Ca2+ 和 Mn2+ 離子存在才能(néng)顯示活性。Con A适用于碳水(shuǐ)化合物(wù)研究，糖蛋白(bái)純化，酶标記（enzyme tagging），細胞膜研究，細胞凝集，和細胞分型研究。

本品是FITC标記的刀豆蛋白(bái)（FITC labeled Concanavalin A, FITC-ConA），Ex/Em=495/515nm，親和純化所得，基本不含未标記的熒光(guāng)素。建議工(gōng)作濃度範圍是5-20μg/ml。

産品特性

1）   英文同義名：Concanavalin A from Canavalia ensiformis (Jack bean), FITC Conjugate; FITC-ConA;

FITC labeled Concanavalin A (ConA); ConA lectin (FITC);

2）   中文同義名：刀豆蛋白(bái) A 來源于洋刀豆 （刀豆），FITC标記；FITC标記的刀豆凝集素A；FITC标記的刀豆球蛋白(bái)A；FITC标記的伴刀豆球蛋白(bái)A；

3）   糖類特異性：α-甘露糖；α-葡萄糖；

4）   F/P（摩爾）：2.0-6.0

5）   外觀：溶液（溶于10 mM HEPES, 0.15 M NaCl, pH 7.5, containing 0.1 mM Ca2+, 0.08% sodium azide and 0.01mM Mn2+）

6）   蛋白(bái)濃度：5mg/ml

7）   Ex/Em：495/515nm

8）   應用：IF、糖生(shēng)物(wù)學

保存與運輸方法

保存：2-8℃避光(guāng)保存，至少1年(nián)有效。

運輸：冰袋運輸。

注意事(shì)項

1）   本品置于2-8℃長(cháng)期保存可能(néng)會(huì)産生(shēng)沉澱，使用前請37℃溫育數分鍾，之後離心吸取上(shàng)清使用。此操作基本不會(huì)對産生(shēng)性能(néng)造成負影響。

2）   為(wèi)了您的安全和健康，請穿實驗服并戴一(yī)次性手套操作。 

常見(jiàn)問題

1）   FITC标記刀豆蛋白(bái)（FITC-ConA）（# MP6321-5MG）的建議工(gōng)作濃度是多(duō)少？ 如何配制染色工(gōng)作液？

回複：本品用于免疫熒光(guāng)染色（IF）的建議工(gōng)作濃度是5-20μg/ml，用戶可根據自(zì)身的實際需求或參考文獻來進行工(gōng)作濃度的優化。可用生(shēng)理緩沖液如PBS，HEPES等稀釋到(dào)工(gōng)作濃度。

2）   FITC标記刀豆蛋白(bái)（FITC-ConA）（# MP6321-5MG）進行免疫熒光(guāng)染色（IF）的建議孵育條件(jiàn)如何？

回複：一(yī)般孵育條件(jiàn)是室溫（RT）孵育30min，也可于4℃孵育30-1h。具體的孵育條件(jiàn)請根據自(zì)身的實際需求或參考文獻來進行優化。

相(xiàng)關文獻a）The Candida albicans ENO1 gene encodes a transglutaminase involved in growth, cell division, morphogenesis, and osmotic protection. PMID: 29386353. 【Cells were washed twice with 1× PBS, permeabilized with 70% ethanol, incubated with 2 μg/ml FITC-ConA in 1× PBS for 30 min at room temperature, washed twice with 1× PBS, and analyzed in the flow cytometer. The maximum excitation wavelength was 495 nm, and the maximum emission wavelength was 515 nm.】

相(xiàng)關文獻b）Staining of the inner acrosomal membrane of human spermatozoa with concanavalin A lectin as an indicator of potential egg penetration ability. PMID: 1718787. 【Spermatozoa were pelleted by microcentrifugation, and excess fixative was removed. The pellet was resuspended in a small volume of fixative remaining and 20μl aliquots of fixed sperm suspension were allowed to air dry on poly-L-lysine-treated slides. The slides were then rinsed in 0.2 M glycine followed by a second wash in 10 mM phosphate buffered saline (PBS). Spermatozoa were labeled with 25μl of 100μg FITC-ConA/mL in PBS for 25 minutes at RT. Slides were rinsed with excess PBS and mounted in medium16 before immediate examination at a magnification of 400X under an Olympus BHT microscope.】

3）   FITC标記刀豆蛋白(bái)（FITC-ConA）（# MP6321-5MG）如何進行視網膜血管染色？

回複：很多(duō)臨床前實驗表明在各種FITC标記的凝集素中刀豆蛋白(bái)A對視網膜血管（retinal vasculature）能(néng)表現出最均勻的染色結果。很多(duō)文獻用FITC-ConA來标記血管壁上(shàng)粘附的白(bái)細胞和血管内皮細胞。使用方法可參考以下(xià)文獻：

文獻a）Simvastatin Inhibits Leukocyte Accumulation and Vascular Permeability in the Retinas of Rats with Streptozotocin-Induced Diabetes. PMID: 15111316.【Six different rats were used in each group. After the induction of deep anesthesia, the chest cavity was carefully opened and a 20-gauge perfusion cannula was introduced into the aorta. Drainage was achieved by opening the right atrium. The animals were then perfused with 100 ml of phosphate-buffered saline (PBS) to wash out blood cells in the vessels, such as erythrocytes and nonadherent leukocytes. After PBS perfusion, the animals were perfused with 25 ml of fluorescein isothiocyanate (FITC)-labeled concanavalin A lectin (ConA; 40 μg/ml in PBS, pH 7.4). ConA was used to label leukocytes adherent to the vessel walls. Residual unbound ConA was removed with PBS perfusion. The retina was carefully removed and fixed with 1% paraformaldehyde, and flat mounts were prepared using a fluorescence anti-fading medium. The retinas were then observed using fluorescence microscopy】

文獻b）VEGF164 Is Proinflammatory in the Diabetic Retina. PMID: 12714656 【Perfusion with FITC-ConA or Rhodamine-Con A (40 μg/mL in PBS [pH 7.4], 5 mg/kg BW) was then performed to label adherent leukocytes and vascular endothelial cells, followed by removal of residual unbound lectin with PBS perfusion.】

 — —Written/Edited by V. Shallan【版權歸MKBio懋康所有】

上海夢澤生物科技有限公司是一(yī)家涉足于生(shēng)命科學和生(shēng)物(wù)技(jì)術(shù)領域研究的試劑、儀器(qì)和實驗室消耗品與實驗服務工(gōng)作，主要從(cóng)事(shì)細胞生(shēng)物(wù)學、植物(wù)學、分子生(shēng)物(wù)學、免疫學、生(shēng)物(wù)化學、蛋白(bái)組學。生(shēng)物(wù)制藥與診斷試劑研發生(shēng)産等領域。 本公司秉承“以人為(wèi)本，以誠為(wèi)信、合同守信”的經營理念。堅持"品質保障"的原則為(wèi)廣大客戶提供優質産品。