【務必注意】：初次使用離子探針的用戶，強烈建議配合：Pluronic F-127, Cell Culture Tested 細胞培養級（MS4301-1G）一(yī)起使用，以提高(gāo)探針的水(shuǐ)溶性和胞内加載性。 

産品标簽

PBFI AM 鉀離子指示劑/探，SBFI AM 鈉離子指示劑/探針，Asante Potassium Green-2，Valinomycin 缬氨黴素，CAS NO：124549-23-1

産品信息: 現貨供應，歡迎咨詢。

産品描述

PBFI，英文全名Potassium-binding Benzofuran Isophthalate，一(yī)種K+敏感的熒光(guāng)探針，用來測定細胞和細胞内區隔（Intracellular compartments）的K+水(shuǐ)平變化。雖然PBFI對K+的選擇能(néng)力弱于Ca2+指示劑比如Fura-2，但在其他一(yī)價陽離子存在體系中，PBFI足以檢測K+的生(shēng)理濃度。結合離子後的PBFI光(guāng)譜變化可通(tōng)過激發光(guāng)比率測定來分析，其能(néng)與使用相(xiàng)同光(guāng)濾片和儀器(qì)檢測的探針Fura-2共同使用。

PBFI對K+的解離常數（Kd）非常依賴于Na+的存在與否。在不含Na+的體系，PBFI對K+的Kd值為(wèi)5.1mM；而在含135mM K+/ Na+總濃度（約為(wèi)生(shēng)理離子強度）的溶液體系，PBFI對K+的Kd值為(wèi)44mM；若緩沖液中的Na+用四甲基氯化铵所替代，PBFI對K+的Kd值變為(wèi)11mM。氯化膽堿和N-甲基葡萄糖胺是培養基中另兩種可能(néng)替代Na+的化合物(wù)。雖然PBFI對K+的選擇性比Na+僅強1.5倍，這一(yī)選擇能(néng)力已足以滿足檢測需求，因為(wèi)正常情況細胞内K+濃度比Na+高(gāo)10倍左右。

本品為(wèi)乙酰氧基甲基酯（Acetoxymethyl ester, AM ester）形式的PBFI，CAS NO：124549-23-1，具有細胞膜滲透性，隻需簡單孵育即可進入細胞，常用加載濃度範圍5-10µM，加載時間40min-4h，根據具體的實驗要求和細胞類型來調整。

産品特性

1） 化學名：4,4'-[1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diylbis(5-methoxy-6,2-benzofurandiyl)]bis- 1,3-benzenedicarboxylic acid, tetrakis[(acetyloxy)methyl] ester

2） 同義名：Potassium-binding Benzofuran Isophthalate Acetoxymethyl ester

3） CAS NO：124549-23-1

4） 分子式：C58H62N2O24

5） 分子量：1171.1

6） 純度：≥95%

7） Ex/Em：~340,380/500 nm

8） 外觀：黃色至橙色粉末

9） 溶解性：溶于DMSO（10mM）和甲醇

10）化學結構式：

保存與運輸方法

保存：-20ºC避光(guāng)幹燥保存，2年(nián)有效。

運輸：冰袋運輸。

注意事(shì)項

1） PBFI AM易受潮，粉末需幹燥保存；粉末需用無水(shuǐ)DMSO溶解，配制儲存液（如10mM），置于-20℃幹燥避光(guāng)保存，小(xiǎo)量分裝避免反複凍融，至少3個(gè)月(yuè)穩定。

2） PBFI AM由于水(shuǐ)溶性較差，建議使用Pluronic F-127以優化探針的細胞加載效率。通(tōng)常情況，将PBFI AM的DMSO儲存液與等體積Pluronic F-127（25% w/v）混合均勻，之後即刻加入适量的細胞加載緩沖液（cell loading buffer）中達到(dào)所需濃度。

3） 為(wèi)了您的安全和健康，請穿實驗服并戴一(yī)次性手套操作。

 — —Written/Edited by V. Shallan【版權歸MKBio懋康所有】

上海夢澤生物科技有限公司是一(yī)家涉足于生(shēng)命科學和生(shēng)物(wù)技(jì)術(shù)領域研究的試劑、儀器(qì)和實驗室消耗品與實驗服務工(gōng)作，主要從(cóng)事(shì)細胞生(shēng)物(wù)學、植物(wù)學、分子生(shēng)物(wù)學、免疫學、生(shēng)物(wù)化學、蛋白(bái)組學。生(shēng)物(wù)制藥與診斷試劑研發生(shēng)産等領域。 本公司秉承“以人為(wèi)本，以誠為(wèi)信、合同守信”的經營理念。堅持"品質保障"的原則為(wèi)廣大客戶提供優質産品。

文獻引用：

[1]

Tong W et al. Phthalocyanine functionalized poly(glycidyl methacrylate) nano-assemblies for photodynamic inactivation of bacteria. Biomater. Sci., 2019,7, 1905-1918

Sodium-binding benzofuran isophthalate acetoxymethyl ester (SBFI-AM) and Potassium-binding Benzofuran Isophthalate Acetoxymethyl ester (PBFI-AM) were purchased from MKbio. China.

[2]

Li R et al. Biofilm inhibition and mode of action of epigallocatechin gallate against Vibrio mimicus. Food Control, Volume 113, July 2020, 107148

Then PBFI probe (ShangHai MaoKang Bio technology Co., LTD., China) was added and incubated at 37 °C for 90 min. The cells were washed, collected and resuspended with PBS buffer. Aliquots (100 μL) of bacterial suspension were transferred to a Corning 96 well black plate, and 100 μL of various concentrations of EGCG were dispensed in the microtiter plate wells.

[3]

Liu Y, Zhen W, Wang Y, Song S, Zhang H. Na2S2O8 Nanoparticles Trigger Antitumor Immunotherapy through Reactive Oxygen Species Storm and Surge of Tumor Osmolarity. J Am Chem Soc. 2020 Dec 30;142(52):21751-21757. doi: 10.1021/jacs.0c09482. Epub 2020 Dec 18. PMID: 33337859.

4T1 cells were inoculated into glass bottom culture dishes for 24 h. Then, adding PNSO NPs medium solution (80 μg/mL) to continue co-culture for 4 h. The treated 4T1 cells were further incubated with 10

µM Na+ indicator SBFI AM (sodiumbinding benzofuran isophthalate acetoxymethyl ester, MKBio, 90%) in 0.04% Pluronic F-127 (MKBio) and the fluorescence signal was measured by CLSM.

[4] 

Liang Z, Yang Y, Yu G, et al. Engineering aluminum hydroxyphosphate nanoparticles with well-controlled surface property to enhance humoral immune responses as vaccine adjuvants. Biomaterials. 2021 Jun;275:120960. DOI: 10.1016/j.biomaterials.2021.120960.

[5] BMDMs were treated with AAHPs (250 μg/mL) in the presence of LPS at 500 ng/mL for 5 h. Then PBFI AM (Mkbio, Shanghai, China) was added to the cells at the concentration of 10 μM, and cells were incubated at 37 °C for 1h. Triton X-100 (0.2%) treated cells were used as controls. The fluorescence of PBFI AM was measured at the Ex/Em of 340/615 nm. The data were expressed as relative fluorescence intensity (RFI) defined as the fluorescence intensity of AAHPs-treated BMDMs normalized to the intensity of control cells.

[6] Jia Y, Yang B, Shi J, Fang D, Wang Z, Liu Y. Melatonin prevents conjugative transfer of plasmid-mediated antibiotic resistance genes by disrupting proton motive force. Pharmacol Res. 2022 Jan;175:105978. doi: 10.1016/j.phrs.2021.105978. Epub 2021 Nov 21. PMID: 34813930.

As for the detection of intracellular K + concentration, the PBFI-AM (K + indicator) fluorescence dye (Maokangbio, Shanghai, China) labeled cells (10 μM) in the presence of…..