目錄号 | MX4509-100UG | 售價 | 1658.00元 | |||||||||||||||||||||||||
規格 | 2×50μg | 運輸溫度 | 冰袋運輸 | |||||||||||||||||||||||||
其他名稱 | SBFI AM ( Na+ Indicator) 鈉離子指示探針 | 保存溫度 | -20℃避光(guāng)幹燥保存 | |||||||||||||||||||||||||
CAS号 | 129423-53-6 | 有效期 | 2年(nián) | |||||||||||||||||||||||||
應用 | 鈉離子指示劑,熒光(guāng)探針 | 訂購數量 |
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産品簡介: SBFI AM ( Na+ Indicator) 鈉離子指示探針
【務必注意】:初次使用離子探針的用戶,強烈建議配合:Pluronic F-127, Cell Culture Tested 細胞培養級(MS4301-1G)一(yī)起使用,以提高(gāo)探針的水(shuǐ)溶性和胞内加載性。
産品标簽 SBFI AM 鈉離子指示劑/探針,PBFI AM 鉀離子指示劑/探針,SQI-Et (Na) 鈉離子載體,Monensin 莫能(néng)黴素,CAS NO:129423-53-6
産品信息
【溫馨提示】:見(jiàn)我司懋康生(shēng)物(wù)(MKBio)整理的鉀離子(K+)熒光(guāng)探針專題。 【溫馨提示】:見(jiàn)我司懋康生(shēng)物(wù)(MKBio)整理的鈉離子(Na+)熒光(guāng)探針專題。 【溫馨提示】:見(jiàn)我司懋康生(shēng)物(wù)(MKBio)整理的鈣離子(Ca2+)熒光(guāng)探針專題。
産品描述 SBFI,英文全名Sodium-binding Benzofuran Isophthalate,一(yī)種Na+選擇性熒光(guāng)指示劑,可用來預測純化線粒體Na+梯度,檢測胞内Na+水(shuǐ)平,測定細胞Na+外流,以及與其他熒光(guāng)指示劑聯合使用以分析Na+與Ca2+和Mg2+濃度、胞内pH和膜電(diàn)位變化的相(xiàng)關性。雖然SBFI對Na+的選擇能(néng)力弱于Ca2+指示劑比如Fura-2,但在其他單價陽離子存在體系,SBFI足以檢測Na+的生(shēng)理濃度。結合離子後的SBFI光(guāng)譜反應可通(tōng)過激發光(guāng)比率測定來判定,其能(néng)與用相(xiàng)同光(guāng)濾片和儀器(qì)檢測的探針Fura-2共同使用。
當體系内含生(shēng)理濃度的K+/Na+(~135mM),SBFI對Na+的解離常數(Kd)為(wèi)11.3mM;而不存在K+體系,對Na+的Kd為(wèi)3.8mM。SBFI對Na+的選擇性比K+約強18倍。
本品為(wèi)乙酰氧基甲基酯(Acetoxymethyl ester, AM ester)形式的SBFI,CAS NO:129423-53-6,具有細胞膜滲透性,隻需簡單孵育即可進入細胞,常用加載濃度範圍5-10µM,加載時間40min-4h,根據具體的實驗要求和細胞類型來調整。
産品特性 1)化學名:4,4’-[1,4,10-trioxa-7,13-diazacyclopentadecane-7,13-diylbis(5-methoxy-6,2-benzofurandiyl)]bis-1,3- benzenedicarboxylic acid 1,1’,3,3’-tetrakis[(acetyloxy)methyl] ester 2)同義名:Sodium-binding Benzofuran Isophthalate Acetoxymethyl ester 3)CAS NO:129423-53-6 4)分子式:C56H58N2O23 5)分子量:1127.07 6)純度:>90%(HPLC) 7)Ex/Em:340,380/500 nm 8)外觀:淺黃至暗(àn)黃至暗(àn)橙固體或油狀 9)溶解性:溶于DMSO(10mM) 10)化學結構式:
保存與運輸方法 保存:-20℃避光(guāng)幹燥穩定凍存二年(nián)。 運輸:冰袋運輸。
注意事(shì)項 1)SBFI AM易受潮,粉末需要幹燥保存;需用無水(shuǐ)DMSO溶解,配制儲存液(如10mM),置于-20℃幹燥避光(guāng),小(xiǎo)量分裝避免反複凍融,至少3個(gè)月(yuè)穩定。 2)由于SBFI AM水(shuǐ)溶性較差,可在實驗前與等體積Pluronic F-127(25% w/v)混合,以提高(gāo)探針加載效率。 3)為(wèi)了您的安全和健康,請穿實驗服并戴一(yī)次性手套操作。
— —Written/Edited by V. Shallan【版權歸MKBio懋康所有】
上海夢澤生物科技有限公司是一(yī)家涉足于生(shēng)命科學和生(shēng)物(wù)技(jì)術(shù)領域研究的試劑、儀器(qì)和實驗室消耗品與實驗服務工(gōng)作,主要從(cóng)事(shì)細胞生(shēng)物(wù)學、植物(wù)學、分子生(shēng)物(wù)學、免疫學、生(shēng)物(wù)化學、蛋白(bái)組學。生(shēng)物(wù)制藥與診斷試劑研發生(shēng)産等領域。 本公司秉承“以人為(wèi)本,以誠為(wèi)信、合同守信”的經營理念。堅持"品質保障"的原則為(wèi)廣大客戶提供優質産品。
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Sodium-binding benzofuran isophthalate acetoxymethyl ester (SBFI-AM) and Potassium-binding Benzofuran Isophthalate Acetoxymethyl ester (PBFI-AM) were purchased from MKbio. China.
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Then PBFI probe (ShangHai MaoKang Bio technology Co., LTD., China) was added and incubated at 37 °C for 90 min. The cells were washed, collected and resuspended with PBS buffer. Aliquots (100 μL) of bacterial suspension were transferred to a Corning 96 well black plate, and 100 μL of various concentrations of EGCG were dispensed in the microtiter plate wells.
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4T1 cells were inoculated into glass bottom culture dishes for 24 h. Then, adding PNSO NPs medium solution (80 μg/mL) to continue co-culture for 4 h. The treated 4T1 cells were further incubated with 10 µM Na+ indicator SBFI AM (sodiumbinding benzofuran isophthalate acetoxymethyl ester, MKBio, 90%) in 0.04% Pluronic F-127 (MKBio) and the fluorescence signal was measured by CLSM.
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[5] BMDMs were treated with AAHPs (250 μg/mL) in the presence of LPS at 500 ng/mL for 5 h. Then PBFI AM (Mkbio, Shanghai, China) was added to the cells at the concentration of 10 μM, and cells were incubated at 37 °C for 1h. Triton X-100 (0.2%) treated cells were used as controls. The fluorescence of PBFI AM was measured at the Ex/Em of 340/615 nm. The data were expressed as relative fluorescence intensity (RFI) defined as the fluorescence intensity of AAHPs-treated BMDMs normalized to the intensity of control cells.
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As for the detection of intracellular K + concentration, the PBFI-AM (K + indicator) fluorescence dye (Maokangbio, Shanghai, China) labeled cells (10 μM) in the presence of…..
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