Fc Receptor Blocking Solution, Human 人Fc受體阻斷劑

産品标簽

Fc Blocker；FcγR blocker；TruStain FcX™；Red Blood Cell Lysis Buffer；7-AAD；Flow cytometry（FC）；

産品信息

溫馨提示：見(jiàn)我司整理的細胞表面抗原免疫熒光(guāng)染色技(jì)術(shù)（Cell Surface Immunofluorescence Staining Protocol）專題。

産品描述

Fc受體（Fc receptors，FcRs）表達于多(duō)種細胞中，比如單核細胞，粒細胞，B細胞和樹突狀細胞。FcR表達細胞在免疫熒光(guāng)染色中有時出現假陽性或假陰性結果，原因在于FcRs介導的Ig Fc結合性。

人Fc受體阻斷劑（Fc Receptor Blocking Solution, Human）特别開(kāi)發用于阻斷FcR引起的非特異性染色，且不會(huì)幹擾抗體介導的人細胞特異性染色。本品與抗人CD16（克隆3G8），CD32（克隆FUN-2）和CD64（克隆10.1）抗體的流式染色兼容。

産品特性

注意事(shì)項

1）建議本品開(kāi)瓶後6個(gè)月(yuè)内用完或在标簽上(shàng)标注日期前用完。

2）本品含疊氮鈉（有毒物(wù)質），請注意防護，避免與皮膚、眼睛和黏膜的接觸。

3）為(wèi)了您的安全和健康，請穿實驗服并戴一(yī)次性手套操作。

引用文獻

【1】Wang S, Peng Y, Wang R, et al. Characterization of neutralizing antibody with prophylactic and therapeutic efficacy against SARS-CoV-2 in rhesus monkeys. Nature Communications. 2020 Nov;11(1):5752. DOI: 10.1038/s41467-020-19568-1.

Cells were collected and washed twice with 1× PBS, and then blocked with Fc receptor blocking solution buffer (Cat: MX1505, Maokang Biological) for 30 min at RT. Then 10 μl anti-FcγRI antibody-FITC anti-FcγRIIa antibody-FITC, anti-FcγRIIIa antibody-FITC, and FITC-labeled anti-FcγRIIb antibody were added into cells (1 × 106 cells/sample in 100 μl) and incubated for 60 min at 2–6 °C and analyzed using flow cytometry. CytoExpert software was used for data analysis.

[2]Hou S, Hao J, Wang YY, Zhao BB, Xiao GW, Li YQ, Liu X, Zou ZL, Yao Y, Xiong H. EBF1 gene promotes the proliferation and inhibits the apoptosis of bone marrow CD34+ cells in patients with myelodysplastic syndrome through negative regulation of mitogen-activated protein kinase axis. J Cell Biochem. 2018 Oct 18. doi: 10.1002/jcb.27177. Epub ahead of print. PMID: 30335886.

Subsequently, 100 μL of an Fc receptor blocking reagent (Shanghai Maokang Biotechnology Co, Ltd, Shanghai, China) was then added into the suspension to eliminate any and all nonspecific binding. After thorough mixing with the blocking reagent

 — —Written/Edited by V. Shallan【版權歸MKBio懋康所有】

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