目錄号 | C7000 | 售價 | 3690.00元 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
規格 | 10×50μg | 運輸溫度 | 室溫 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
其他名稱 | CellTracker™ CM-DiI | 保存溫度 | -20℃避光(guāng)保存 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
CAS号 | 180854-97-1 | 有效期 | 1年(nián) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
應用 | 活細胞染色劑 | 訂購數量 |
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産品簡介: CellTracker™ CM-DiI 活細胞示蹤劑
訂購信息:【原裝産品,現貨提供】
基本介紹: CM-DiI(活細胞的多(duō)代示蹤、固定細胞長(cháng)期示蹤) CellTracker CM-DiI為(wèi)DiI的衍生(shēng)物(wù),在DiI中加入了具有中度巯基反應特性的氯甲基取代物(wù),後者能(néng)與細胞内含巯基的肽和蛋白(bái)結合,使得CM-DiI能(néng)夠抵抗醛類的固定作用。CM-DiI在細胞固定、破膜及石蠟包埋的整個(gè)過程中均能(néng)很好的保留在細胞内,因此,與傳統細胞膜探針DiI相(xiàng)比較,更适于固定後标本的長(cháng)期示蹤。其紅(hóng)色/綠色的光(guāng)譜特征,使其可與綠色探針或者綠色熒光(guāng)蛋白(bái)如GFP一(yī)起用于雙标實驗。
産品特性 1)CAS NO:180854-97-1 2)化學名:3H-Indolium, 5-[[[4-(chloromethyl)benzoyl]amino]methyl]-2-[3-(1,3-dihydro-3,3-dimethyl-1- octadecyl-2H-indol-2-ylidene)-1-propenyl]-3,3-dimethyl-1-octadecyl-, chloride 3)分子式:C68H105Cl2N3O 4)分子量:1051.5g/mol 5) 純度:≥95% 6) 外觀:紅(hóng)色固體 7) Ex/Em:553/570 nm(甲醇) 8) 推薦濾光(guāng)器(qì):Omega-XF32, Chroma-31002 9) 溶解性:溶于DMSO,乙醇,DMF 10)化學結構圖:
染色示例
Fig 1.Brain of a zebrafish embryo. CellTracker CM-DiI. A 30 µm–thick section of a zebrafish embryo stained with CellTracker CM-DiI (C7000, C7001) prior to immunohistochemical analysis. A one-day-old zebrafish embryo was immobilized and impaled in the hindbrain with a microelectrode filled with 1 mg/mL CellTracker CM-DiI in 95% ethanol. One day later, the brain was fixed, embedded, frozen in liquid nitrogen and sectioned on a cryostat. After blocking with 0.1% Triton X-100 in PBS containing 2% BSA and 2% normal goat serum, the 30 µm–thick section was incubated with primary anti-glial antibody in conjunction with fluorescein goat anti–mouse IgG antibody. This section was then viewed sequentially through optical filter sets appropriate for rhodamine and fluorescein, and the resulting images were superimposed. The image was contributed by William Trevarrow, Beckman Institute, California Institute of Technology.
細胞膜熒光(guāng)探針系列産品:【見(jiàn)整理專題】 1. 常見(jiàn)細胞膜探針和檢測光(guāng)譜
2. 常見(jiàn)細胞膜探針訂購信息
— —Written/Edited by V. Shallan【版權歸MKBio懋康所有】
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