Plant Preservative Mixture (PPM) 植物(wù)組培抗菌劑

——植物(wù)培養防腐劑（抗菌劑）/生(shēng)物(wù)殺滅劑/農藥

關鍵詞：植物(wù)培養抗菌劑（plant culture preservative），農藥（biocide），抗真菌劑，抑制農杆菌生(shēng)長(cháng)；

作用機(jī)制：

PPM（Plant Preservative Mixture，植物(wù)組培抗菌劑）是用于植物(wù)細胞培養中的一(yī)種強效且廣譜的生(shēng)物(wù)殺滅劑，由多(duō)種抗菌劑組合而成的混合物(wù)，能(néng)殺死植物(wù)組培培養基和污染組織内的細菌和真菌，作用機(jī)制在于能(néng)夠穿透細菌或真菌細胞壁，抑制檸檬酸循環和電(diàn)子傳遞鏈等中心代謝循環中關鍵酶的生(shēng)物(wù)活性，以及抑制培養基内單糖和氨基酸往細菌或者真菌細胞的運輸。取決于PPM劑量和污染程度，PPM可用作植物(wù)培養基内的生(shēng)物(wù)殺滅劑組分。PPM還(hái)可用作生(shēng)物(wù)靜(jìng)力化合物(wù)起到(dào)預防效果。除此之外，PPM能(néng)有效抑制植物(wù)培養液體或半固體培養中通(tōng)過空氣、水(shuǐ)、人傳播的微生(shēng)物(wù)污染以及内源性污染，但不會(huì)影響體外種子發芽、愈傷組織生(shēng)長(cháng)以及根莖再生(shēng)。

适用植物(wù)類型：

PPM有效作用于大多(duō)數種子裸露植物(wù)（被子植物(wù)和裸子植物(wù)），但不适合用于蕨類，藓類，藻類和水(shuǐ)生(shēng)植物(wù)。【PPM是一(yī)種非常有效的抗微生(shēng)物(wù)劑以預防和去除組培污染，然而不能(néng)替代實驗室的無菌處理試劑，建議使用合适的空氣無菌處理系統。】

産品優勢【與傳統的植物(wù)組培抗微生(shēng)物(wù)劑相(xiàng)比】：

1）适當濃度下(xià)不會(huì)影響大多(duō)數植物(wù)生(shēng)長(cháng)；2）極低(dī)濃度即可維持無菌的培養環境；3）可預處理新生(shēng)種子或清洗組織；4）熱穩定性強，高(gāo)壓滅菌非常穩定；5）比傳統抗生(shēng)素價格更低(dī)廉，可作為(wèi)組培培養基内的标準成分，并作為(wèi)常規使用；6）靶向抑制多(duō)種酶活性，植株産生(shēng)耐藥性的幾率非常低(dī)；

産品特性：

外觀：清澈，透明至琥珀色溶液，pH 3.8

保存和穩定性：4°C，3年(nián)有效

使用特點：1）PPM可在滅菌前加入培養基或者分液前直接加入滅菌培養基；

          2）PPM可在壓力15 psi（1.05 kg/cm²），121°C滅菌20min；

          3）PPM需要滅菌後（post-autoclave）後加入含蛋白(bái)質的培養基内，之後将混合好的培養基倒入培養皿；

4）工(gōng)作濃度的PPM（經培養基稀釋）可室溫穩定存放(fàng)高(gāo)達1個(gè)月(yuè)；

使用濃度參考：

産品訂購：更大包裝或産品技(jì)術(shù)問題，請來電(diàn)/在線咨詢，電(diàn)話：021-54736159 。 網站：www.maokangbio.com。

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