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Polyethylenimine,Linear (PEI) 線性化聚乙烯亞胺産品專題
時間:2016-12-02     作者:懋康生(shēng)物(wù)     文章來源:懋康原創    

品牌故事(shì)— —我的故事(shì)裡(lǐ)怎能(néng)少了你?


Polysciences,Inc,成立于1961年(nián),起家于化學但超越化學領域的美國(guó)制造商,總部位于賓夕法尼亞州沃靈頓,公司業(yè)務主要涵蓋三大塊:1.實驗用産品:a)成千上(shàng)萬種具品牌特色的化合物(wù)和試劑,應用于實驗室研究,組織學,電(diàn)鏡和解剖病理學以及其他診斷和生(shēng)命科學研究;b)種類極其寬廣的單體和聚合體,滿足各方研究領域;c)有機(jī)/無機(jī)化合物(wù),生(shēng)物(wù)可降解材料,磁珠,熒光(guāng)素,染料和特定抗體,以及常規蛋白(bái)包被顆粒(直徑範圍40nm~10mm)的全球首要制造工(gōng)廠;d)頂端多(duō)聚化合物(wù)的供貨中心,滿足各種高(gāo)性能(néng)工(gōng)業(yè)領域需求,包括替代能(néng)源,汽車電(diàn)子,國(guó)防&航空電(diàn)子,便攜式通(tōng)信和計算(suàn)機(jī),醫(yī)療器(qì)械組裝。2.定制合成;3.cGMP生(shēng)産服務。

全球聯系方式:1(800)523-2575;(215)343-6484。


Brand Story:

Me,Polysciences,Inc,Chemistry beyond the ordinary

Me,Polysciences,Inc,Headquartered at Warrington,PA,Call vial 1(800)523-2575

Me,Polysciences,Inc,Manufacturing hundreds of specialty chemicals and reagents for laboratory research, Histology, Light Microscopy and Anatomic Pathology as well as other clinical and life science applications.

Me,Polysciences,Inc,Stocking a wide portfolio of monomers and polymers for research applications.

Me,Polysciences,Inc,Developed to be the primary source for organic, inorganic, biodegradable, magnetic, fluorescent, dyed and specific antibody and general protein coated particles in diameters spanning the range of 40nm to 10mm.

Me,Polysciences,Inc,Offering innovative polymer products to meet the high performance requirements of a variety of industries including alternative energy, automotive electronics, defense & aerospace electronics, portable communications & computing, medical device assembly, and more. 

— — Written/Edited by V. Shallan

 

Special Products:

你會(huì)喜歡的第二代聚合物(wù)轉染試劑

Polyethylenimine,Linear,Mw 25,000 線性化聚乙烯亞胺(PEI 25,000)


基本描述:

線性化聚乙烯亞胺PEI 25,000,一(yī)種高(gāo)電(diàn)荷陽離子聚合物(wù),非常容易結合于高(gāo)電(diàn)荷陰離子基質。工(gōng)業(yè)應用上(shàng),線性化PEI可改善負電(diàn)荷染料的物(wù)理外觀,以調整染料的化學特性和增強染料與固相(xiàng)基質的黏附能(néng)力,常用作黏附增強劑,作用同多(duō)聚賴氨酸(poly-lysine);科研應用上(shàng),線性化PEI能(néng)與DNA或其他負電(diàn)荷生(shēng)物(wù)大分子簡單結合,正是這一(yī)特性,使得成為(wèi)一(yī)種非常行之有效的載體運輸介質(Vector carrier),即轉染試劑


化學性質:

CAS NO.                      

9002-98-6, 26913-06-4                                                                     

Formula

(CH2CH2NH)n

Molecular Weight              

25,000

Melting Point

73-75º 

Solubility

Soluble In:hot water, cold water at low pH, methanol, and ethanol.

Insoluble In: benzene, ethyl ether and acetone

Appearance

White to yellow solid



訂購信息:【原裝進口,現貨供應,轉染級别Transfection Grade】 

品牌

貨号

名稱

規格       

促銷價(元)

Polysciences

23966-1      

線性聚乙烯亞胺(MW 25,000) 

1g

16180

Polysciences

23966-100

線性聚乙烯亞胺(MW 25,000)

100mg

2310

另,提供更高(gāo)轉化效率的PEI Max 40,000,自(zì)由基形式分子量相(xiàng)當于PEI 22,000,研究證實其

體外質粒DNA轉染效率提高(gāo)達21倍。

Polysciences

24765-1

線性聚乙烯亞胺鹽酸鹽Max,(MW 40,000)

(Equivalent to Mw 22,000 in Free Base Form)   

1g

16655

Polysciences   

24765-100

線性聚乙烯亞胺鹽酸鹽Max,(MW 40,000)

(Equivalent to Mw 22,000 in Free Base Form)

100mg

2502

【好消息】:針對小(xiǎo)量用戶或初次使用用戶,現針對24765-2g,提供250mg小(xiǎo)包裝,目錄價948元;和1g 包裝,目錄價3000元。歡迎來電(diàn)咨詢具體價格。


使用方法【來源于:CSH protocols,僅作交流學習】:

1. Pour ~450 mL of Milli-Q H2O into a 500-mL glass beaker.

2. Add 500 mg of PEI to the beaker with stirring.

3. Add concentrated HCl drop-wise to the solution to pH <2.0.

4. Stir until the PEI is dissolved (~2-3 h). Maintain the pH <2.0 throughout.

Approximately 800 μL of 12 M HCl will be required for full PEI dissolution. There may still be some small fiber particles that will not dissolve.

5. Add concentrated NaOH drop-wise to the solution to pH 7.0.

Approximately 500 μL of 10 M NaOH will be required to neutralize the PEI solution.

6. Pour the solution into a 500-mL glass cylinder. Adjust the final volume to 500 mL with Milli-Q H2O.

7. Filter-sterilize the solution through a 0.22-μm membrane.

8. Store aliquots of the desired volume at -20°C.


應用文獻【基因轉染用途】:

Zhong Z, Feijen J, Lok MC, Hennink WE, Christensen LV, Yockman JW, Kim YH, Kim SW. (2005). Low molecular weight linear polyethylenimine-b-poly(ethylene glycol)-b-polyethylenimine triblock copolymers: synthesis, characterization, and in vitro gene transfer properties.Biomacromolecules. 6(6):3440-8.

Sharon E. Reed, Elizabeth M. Staley, John P. Mayginnes, David J. Pintel(2006). Transfection of mammalian cells using linear polyethylenimine is a simple and effective means of producing recombinant adeno-associated virus vectors.Journal of Virological Methods.138(1–2): 85–98.

Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy(2013)Transient Mammalian Cell Transfection with Polyethylenimine (PEI).Methods Enzymol. 529: 227–240.


— — Written/Edited by V. Shallan【版權歸MKBio懋康所有】


更高(gāo)效的PEI聚乙烯亞胺聚合物(wù)轉染試劑

Polyethylenimine HCl MAX, Linear, Mw 40,000 線性化聚乙烯亞胺鹽酸鹽MAX(PEI 40,000)


基本描述:

Polyethylenimine HCl MAX, Linear, Mw 40,000(PEI 40,000)是熱賣産品線性化聚乙烯亞胺PEI25,000的升級産品,不同之處在于1)完全水(shuǐ)解(去乙酰化);2)PEI25,000的鹽酸鹽形式,具更高(gāo)的水(shuǐ)溶特性;3)含更長(cháng)連續性乙烯亞胺(ethyleneimine)片段,其質子化氮水(shuǐ)平比PEI25,000提高(gāo)11%以上(shàng)。本品經堿中和後,可轉化為(wèi)線性化自(zì)由胺(PEI),此時分子量正相(xiàng)當于25,000。

Thomas M,et al(2005)研究數據表明,去乙酰化的PEI 40,000(Polyethylenimine MAX 40,000)體外質粒DNA轉染效率提高(gāo)21倍,小(xiǎo)鼠體内靶向效率達到(dào)10,000倍,随之肺部特異療效顯示增強1500倍。


化學性質:

CAS NO.                      

9002-98-6, 26913-06-4

Molecular Weight

40,000(~22,000 free base)

Solubility

Soluble In:Cold and room temperature water 

Insoluble In: Common organic solvents (ethanol, acetone, tetrahydrofuran)

Appearance

White to off-white free flowing solid



訂購信息:【原裝進口,現貨供應,轉染級别Transfection Grade

品牌

貨号

名稱

規格        

促銷價(元)

Polysciences   

24765-1   

線性聚乙烯亞胺鹽酸鹽Max,(MW 40,000)

(Equivalent to Mw 22,000 in Free Base Form)      

1g

16180

Polysciences

24765-100

線性聚乙烯亞胺鹽酸鹽Max,(MW 40,000)

(Equivalent to Mw 22,000 in Free Base Form)

100mg

2310

另,提供基本型PEI 25,000。

Polysciences

23966-1

線性聚乙烯亞胺(MW 25,000)

1g

16655

Polysciences

23966-100

線性聚乙烯亞胺(MW 25,000)

100mg

2502

【好消息】:針對小(xiǎo)量用戶或初次使用用戶,現針對24765-2g,提供250mg小(xiǎo)包裝,目錄價948元;和1g 包裝,目錄價3000元。歡迎來電(diàn)咨詢具體價格。


 應用文獻【基因轉染用途】:

Mann JF, McKay PF, Arokiasamy S, Patel RK, Klein K, Shattock RJ. (2013). Pulmonary delivery of DNA vaccine constructs using deacylated PEI elicits immune responses and protects against viral challenge infection. J Control Release. 170(3):452-9.

Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci U S A. 102(16):5679-84.

 

— — Written/Edited by V. Shallan【版權歸MKBio懋康所有】


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