Rhod-2, AM, Cell Permeant 鈣離子熒光(guāng)探針,超級純
n 最長(cháng)波長(cháng)的Ca2+熒光(guāng)探針
搜索關鍵詞:Rhod-2, AM, Cell Permeant 鈣離子熒光(guāng)探針,超級純;Fluo-3, AM; Fluo-3, Acetoxymethyl Ester; 可見(jiàn)光(guāng)激發波長(cháng)Ca2+熒光(guāng)探針;Fluo-4;Fluo-8, AM;CAS NO:129787-64-0;
Rhod-2是一(yī)種高(gāo)親和力的可見(jiàn)光(guāng)激發波長(cháng)Ca2+熒光(guāng)探針,波長(cháng)明顯長(cháng)于Fluo-3或Fluo-4,其與Ca2+結合後的最大激發波長(cháng)為(wèi)552nm,最大發射波長(cháng)為(wèi)581nm,這類探針更适用于具高(gāo)水(shuǐ)平自(zì)熒光(guāng)信号的細胞或組織Ca2+水(shuǐ)平檢測,也可用來檢測由光(guāng)感受器(qì)和籠鎖鈣離子螯合劑光(guāng)激活導緻的鈣離子釋放(fàng)。Rhod-2, AM是Rhod-2的一(yī)種乙酰甲酯衍生(shēng)物(wù),具有細胞膜滲透性,隻需簡單培養,即可輕易進入細胞。一(yī)旦進入細胞内,即被其内酯酶剪切生(shēng)成不具膜滲透性的Rhod-2,從(cóng)而滞留在胞内以發揮相(xiàng)應生(shēng)理功能(néng)。
可見(jiàn)光(guāng)激發Ca2+熒光(guāng)探針的優勢:
與紫外光(guāng)激發的熒光(guāng)探針相(xiàng)比,如Fura-2和Indo-1,可見(jiàn)光(guāng)激發Ca2+探針具有以下(xià)特點:
1)适用于大多(duō)數的激光(guāng)共聚焦測鈣系統,包括共聚焦激發掃描顯微鏡以及流式細胞儀等。
2) 具有更強的染料吸收性能(néng),使得更低(dī)濃度的探針即可成功檢測Ca2+變化,從(cóng)而降低(dī)對活細胞的光(guāng)毒性。
3)Ca2+依賴性熒光(guāng)強度增強,對Ca2+變化水(shuǐ)平檢測敏感度更高(gāo)。
4)降低(dī)樣品自(zì)熒光(guāng)以及光(guāng)散射的幹擾。
5)兼容光(guāng)激活探針以及UV-激發試劑,因此更方便于多(duō)參數檢測。
6)無光(guāng)譜偏移。
Rhod-2, AM的應用:
Rhod-2,波長(cháng)明顯長(cháng)于Fluo-3/Fluo-4,Ex/Em=552/581nm(Ca2+結合),更适用于具高(gāo)水(shuǐ)平自(zì)熒光(guāng)信号的細胞或組織Ca2+水(shuǐ)平檢測,也可用來檢測由光(guāng)感受器(qì)和籠鎖鈣離子螯合劑光(guāng)激活導緻的鈣離子釋放(fàng)。
Rhod-2, AM的應用實例:
Fig. 1,Metal-ion response screening for Rhod-2, AM. The maximum relative fluorescence intensity was measured for identical indicator concentrations in solutions containing 10 mM EGTA + 10 µM TPEN, 1 µM ion (100 µM for Mg2+) and 100 µM ion (10 mM for Mg2+). Results are plotted as fluorescence changes relative to the ion-free (10 mM EGTA + 10 µM TPEN) reference solution expressed as (F-Fo)/Fo, where F is the fluorescence intensity of ion-containing solutions and Fo is the fluorescence intensity of the reference solution. Blue bars indicate the response to 1 µM ion (100 µM for Mg2+), and red bars indicate the response to 100 µM ion (10 mM for Mg2+).
産品訂購【進口原料,現貨或1周貨期】:
貨号 |
産品名稱 |
Ex/Em(nm) DNA-bound |
規格 |
價格(元) |
MX4507-50UG |
Rhod-2, AM, Cell Permeant 鈣離子熒光(guāng)探針,超級純 |
549/578 |
50μg |
375 |
MX4507-250UG |
549/578 |
5×50μg |
1500 |
相(xiàng)關産品【進口原料,現貨或1周貨期】:
貨号 |
産品名稱 |
Ex/Em(nm) DNA-bound |
規格 |
價格(元) |
MX4503-50UG |
Fluo-3, AM, Cell Permeant 鈣離子熒光(guāng)探針,超級純 |
506/526 |
50μg |
375 |
MX4503-250UG |
506/526 |
5×50μg |
1500 |
|
MX4504-50UG |
Fluo-4, AM, Cell Permeant 鈣離子熒光(guāng)探針,超級純 |
494/516 |
50μg |
375 |
MX4504-250UG |
494/516 |
5×50μg |
1500 |
|
MX4505-50UG |
Fluo-8 AM, Cell Permeant 鈣離子熒光(guāng)探針,超級純 |
490/514 |
50μg |
|
MX4505-250UG |
490/514 |
5×50μg |
|
|
MX4507-50UG |
Rhod-2, AM, Cell Permeant 鈣離子熒光(guāng)探針,超級純 |
549/578 |
50μg |
375 |
MX4507-250UG |
549/578 |
5×50μg |
1500 |
— —Written/Edited by V. Shallan【版權歸MKBio懋康所有】