DiSC3(5) (3,3'-Dipropylthiadicarbocyanine Iodide)

關鍵詞：

DiSC3(5);DiOC5(3)；DiOC6(3)；ER-Tracker Blue-White DPX；Membrane depolarization assay膜去極化實驗；CAS：53213-94-8；

訂購信息：

産品描述

DiSC3(5) (3,3'-Dipropylthiadicarbocyanine Iodide)，一(yī)種具短（C3）烷基尾的羰花青染料，這種陽離子染料能(néng)用來檢測和測量由膜改性試劑引起的跨膜電(diàn)位或結構變化。

産品特性

1. CAS NO：53213-94-8

2. 化學名：Benzothiazolium, 3-propyl-2-(5-(3-propyl)-2(3H)-benzothiazolidene-1,3-pentadienyl), iodide

3. 英文同義名：diS-C3-(5); 3,3'-Di-n-propylthiadicarbocyanine iodide; 3,3-Dipropylthiadicarbocyanine iodide; 5-(3-Propylbenzothiazol-2-ylidene)-1-(3-propylbenzothiazolium-2-yl)-1,3-pentadiene Iodide; 3-Propyl-2-[5-[3-propyl-2(3H)-benzothiazolylidene]-1,3-pentadienyl]benzothiazolium iodide;

4. 中文同義名：3,3'-二丙基硫雜(zá)二羰花青碘化物(wù)；碘化-3,3ˊ-二丙基硫雜(zá)二羰花青；

5. 分子式：C25H27IN2S2

6. 分子量：546.53g/mol

7. 純度：≥98%（HPLC）

8.  Ex/Em：500/705 nm (in DMF)

9.  溶解性：溶于DMF

10.   化學結構式： 

保存與運輸方法

保存：室溫避光(guāng)幹燥保存，可置于-20℃長(cháng)期幹燥避光(guāng)保存，2年(nián)有效。

運輸：室溫運輸。

應用示例：

文獻來源：Juba ML et al. Helical cationic antimicrobial peptide length and its impact on membrane disruption. Biochim Biophys Acta. 2015 May;1848(5):1081-91. PMID: 25660753

實驗方法（膜去極化實驗MK）：Frozen aliquots ofenumerated bacteria (E. coli or B. cereus)were thawed on ice and washed 3 times with buffer (5 mM HEPES with 20 mM glucose, pH 7.4). Following washing, the pelleted bacteria were re-suspended in HEPES buffer (5 mM HEPES, pH 7.4, 20 mM glucose) containing either, 10 or 100 mM KCl. A 96-well plate was prepared where wells are charged with360 μL of bacterial suspension (2 × 107 CFU/mL) and 4.19 μL of diSC3-(5) (200 nM) for a total volume of 364.19 μL.The bacteria were incubated at room temperature and fluorescence was monitored (622 nmex/670 nmem) until diSC3-(5) maximal uptake was obtained. Maximal diSC3-(5) uptake is indicated by a baseline in fluorescence due to self-quenching as the dye concentrates in the cell membrane. Peptide (NA-CATH, L- or D-ATRA-1A) was added at varied concentrations (200–2 μg/mL) in 20 μL aliquots and the fluorescence increase due to induced depolarization of the cytoplasmic membrane was recorded. A negative control of bacteria and diSC3-(5) was used as a background. As a positive control, complete collapse of the membrane potential was attained with valinomycin (200–2 μg/mL), a potassium ionophore.

檢測原理（MK-DiSC3-(5)的膜去極化實驗）：DiSC3(5)作為(wèi)一(yī)種膜電(diàn)位敏感探針，聚集在磷脂雙分子層内，引起染料的自(zì)淬滅。當膜改性化合物(wù)（比如多(duō)肽）使膜去極化，電(diàn)位喪失，DiSC3(5)釋放(fàng)進入溶液引起熒光(guāng)增強，熒光(guāng)強弱與電(diàn)位減少程度呈正比。 

結果展現（MK-DiSC3-(5)的膜去極化實驗）：

文獻來源：Te Winkel JDet al.Analysis of Antimicrobial-Triggered Membrane Depolarization Using Voltage Sensitive Dyes. Front Cell Dev Biol. 2016 Apr 13;4:29. PMID: 27148531

實驗方法（膜電(diàn)位的顯微分析MK）：Early-mid logarithmic growth phase cell suspensions were incubated with 2 μM DiSC3(5)directly in the growth medium. The incubation was carried out under shaking for 5 min, immediately followed by microscopy.A final concentration of 0.5–1% DiSC3(5)-solvent dimethyl sulfoxide (DMSO) was found to be crucial in order to maintain appropriate solubility;lower solvent concentrations resulted in a strongly reduced cellular fluorescence. The incubation was carried out at growth temperature, and under vigorous shaking in order to maintain good energization of the cells. This step was routinely carried out with 2 ml round bottom Eppendorf tubes containing 200 μl cell suspensionin a thermomixer. To provide sufficient aeration, the lids of the tubes were perforated. When dissipation of membrane potential was tested, a compound of interest was added in parallel to DiSC3(5).Addition of 5 μM gramicidin (a mixture of gramicidin A, B, C, and D)was routinely used as a positive control. The imaging of DiSC3(5)-stained cells was carried out using commonly availableCy5-filter sets.【重要信息：文章多(duō)次提及到(dào)DiSC3(5)的工(gōng)作濃度内維持1%DMSO濃度，對維持染料的溶解性和熒光(guāng)穩定性很是關鍵。】

結果展現（MK-DiSC3-(5)的單細胞膜電(diàn)位顯微分析）：

注意事(shì)項

1）熒光(guāng)染料均存在淬滅問題，請盡量注意避光(guāng)，以減緩熒光(guāng)淬滅。

2） 為(wèi)了您的安全和健康，請穿實驗服并戴一(yī)次性手套操作。

相(xiàng)關産品：

——Written/Edited by V. Shallan【版權歸MKBio懋康所有】

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