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當前位置: 首頁> 産品中心> 免疫學 > 免疫細胞組織化學分析 > Agarose bound Lycopersicon Esculentum (Tomato) Lectin (LEL, TL)
Agarose bound Lycopersicon Esculentum (Tomato) Lectin (LEL, TL)
目錄号 AL-1173 售價 3800.00元
規格 2mg 運輸溫度 冰袋運輸。
其他名稱 保存溫度 2-8℃幹燥保存
CAS号 N/A 有效期 有效期一(yī)年(nián)
應用 血管功能(néng)研究 訂購數量
産品簡介:

Agarose bound Lycopersicon Esculentum (Tomato) Lectin (LEL, TL)

AL-1173瓊脂糖結合番茄凝集素

 

搜索關鍵詞:

番茄凝集素;血管内灌注;凝集素灌注;微血管;内皮細胞;LEA;TL-1176 ;  VECTOR;  LEL,TL;  LAB

 

産品訂購:【VECTOR】原裝正品,現貨供應,歡迎來電(diàn)咨詢,電(diàn)話:021-54736159,QQ2971634497 ,1563822900.。

品牌

産品名稱

産品編号

規格

價格

VECTOR

Agarose bound Lycopersicon Esculentum (Tomato) Lectin (LEL, TL)

 

AL-1173

2mg

3800

【溫馨提示:見(jiàn)我司懋康生(shēng)物(wù)整理的血管功能(néng)研究标志(zhì)物(wù)-番茄凝集素産品專題】

 

産品描述:

番茄凝集素(Tomato lectin, Lycopersicon Esculentum lectin (LEL)),是一(yī)種非常穩定的單亞基糖蛋白(bái),約含有等量的蛋白(bái)和碳水(shuǐ)化合物(wù)。據報(bào)道其糖類部分由85%半乳糖組成,在溶液中可能(néng)形成多(duō)聚體聚合物(wù)。番茄凝集素雖然和土(tǔ)豆凝集素、曼陀羅凝集素(DSL)和麥胚凝集素(WGA)有部分相(xiàng)同的特異性、但在許多(duō)多(duō)面呈現出獨特之處。番茄凝集素LEL能(néng)夠很好地與血型糖蛋白(bái)(glycophorin)和tamm-Horsfall糖蛋白(bái)結合,能(néng)夠有效結合和标記齧齒類動物(wù)的血管内皮(vascularendothelium)

 

保存與運輸方法:

保存:2-8℃幹燥保存、有效期一(yī)年(nián)

運輸:冰袋運輸。

 

Overview

Features:

Matrix is heat stable, cross-linked 4% agarose beads with a molecular exlusion of about 2x107 daltons

Bead diameter ranges in size from 45-165 microns

Matrix is stable in solutions at pH 3-11 as well as many organic solvents

Immobilized lectins are prepared using affinity purified lectins

Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions

Hydrophilic spacer arm is inserted between the lectin and the matrix

Conjugated proteins are not leached off the beads by Tris or other routinely used buffers

No residual charges present after conjugation.  This minimizes non-specific binding to the matrix

Product supplied as a 1:1 suspension in buffer

2 mg lectin/ml gel

Inhibiting/Eluting Sugar: Chitin Hydrolysate or Glycoprotein Eluting Solution (ES-5100)

 

Cat. No.

AL-1173-2

Unit Size

2 ml

Country of Manufacture

United States

Applications

Glycobiology, Affinity Chromatography

Matrix Conjugate

Lectins

Sugar Specificity

[GlcNAc]1-3, N-Acetylglucosamine

Conjugate

Agarose

 

More Information

Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents.

 

Agarose bound* tomato lectin is prepared using our affinity-purified lectins. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 daltons are used as the solid-phase matrix to which the lectins are covalently coupled. The attachment of the lectins to the beads is carefully controlled to preserve lectin activity and minimize conformational changes of the bound lectins that might result in nonspecific ionic or hydrophobic interactions. The technique we have developed to couple lectins to agarose beads inserts a hydrophilic spacer arm between the lectin and the matrix.

This coupling method provides several advantages over the traditional cyanogen bromide procedure:

Maximum carbohydrate binding activity of the coupled lectins is retained

Linkage is stable over a range of pH values

Conjugated proteins are not leached off the beads by Tris or other routinely used buffers

No residual charges are present after conjugation. This minimizes non-specific binding to the matrix.

Our agarose bound lectins are supplied at a constant concentration of lectin per ml of settled beads. The concentration for each lectin is selected to achieve the highest glycoconjugate binding capacity per mg of lectin present in the beads. Each lot is tested for its binding capacity using glycoproteins known to bind the lectin. This provides a guideline for the user and assures the quality of our agarose bound lectins.

Inhibiting/Eluting Sugar: Chitin Hydrolysate

*2 mg lectin/ml gel

 

注意事(shì)項:

為(wèi)了您的安全和健康,請穿實驗服并戴一(yī)次性手套操作。

 

另我司懋康生(shēng)物(wù)提供自(zì)主品牌MKbio的番茄凝集素産品,質量保證,現貨供應,歡迎來電(diàn)咨詢,電(diàn)話:021-54736159,QQ2971634497 ,1563822900.。

産品名稱

産品編号

規格

目錄價

FITC labeled Lycopersicon

Esculentum(Tomato)Lectin(Ex/Em=495/515nm,綠色熒光(guāng))

MP6311-500UG

500ug

1400

 

 

 

 — —Written/Edited by V. Shallan【版權歸MKBio懋康所有】

 

 

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