産品名稱

産品編号

CAS NO.         

規格            

價格（元）  

Diphenyleneiodonium Chloride (DPI) 二苯基氯化碘鹽      

MZ2701-10MG    

4673-26-1

10mg

988

Diphenyleneiodonium Chloride (DPI) 二苯基氯化碘鹽

MZ2701-50MG

4673-26-1

50mg

2988

         質量          

溶劑體積

濃度

1mg

5mg

10mg

1mM

3.1791 mL        

15.8957 mL     

31.7914 mL    

5mM

0.6358 mL

3.1791 mL

6.3583 mL

10mM

0.3179 mL

0.5403 mL

3.1791 mL

文獻1，Ye C et al. Identification of a novel small-molecule agonist for human G protein-coupled receptor 3. J Pharmacol Exp Ther. 2014 Jun;349(3):437-43.PMID: 24633425

體外研究（細胞實驗）：

細胞類型（Cell type）：U2OS cells

藥物(wù)配制（Preparation）：DPI was dissolved in DMSO to make stock solution and stored at -20℃.

實驗方法（Assay）：U2OS cells were seeded onto glass coverslips in 24-well plates and transfected with plasmids encoding FLAG-GPR3-GFP. Thirty-six hours later, cells were changed into Dulbecco’s modified Eagle’s medium containing 0.5% FBS at 37°C for 2 hours followed by 1-hour incubation in mediacontaining DPI (10 μM) or vehicle [0.1% dimethylsulfoxide (DMSO)].Cells were then washed three times with phosphate-buffered saline and fixed in 4% paraformaldehyde. After counterstaining of nuclei with Hoechst dye 33342, cells were examined using an FV10i confocal microscope.

文獻2，Deng CC et al.Nigericin selectively targets cancer stem cells in nasopharyngeal carcinoma. Int J Biochem Cell Biol. 2013 Sep;45(9):1997-2006. PMID: 23831840

體内研究（動物(wù)模型）：

動物(wù)模型（Animal Model）：ddy mice (6–7 wk of age)

實驗方法（Assay）：The ddy mice (6–7 wk of age) were individually placed in transparent cages for 30 min before experiments.An intraplantar injection of 10 μl DPI (2 mM, solvent: Kolliphor EL with 20% DMSO) was then injected into the right hindpaw with or without intraperitoneal administration with HC030031 (300 mg/kg at 0.5 h prior to injection of DPI; solvent: saline with 0.5% methyl cellulose).The time spent licking or biting the injected paw was recorded for 45 min after injection.