Polyethylenimine，Linear，Mw 25，000 線性化聚乙烯亞胺（PEI 25，000）

基本描述：

線性化聚乙烯亞胺PEI 25，000，一(yī)種高(gāo)電(diàn)荷陽離子聚合物(wù)，非常容易結合于高(gāo)電(diàn)荷陰離子基質。工(gōng)業(yè)應用上(shàng)，線性化PEI可改善負電(diàn)荷染料的物(wù)理外觀，以調整染料的化學特性和增強染料與固相(xiàng)基質的黏附能(néng)力，常用作黏附增強劑，作用同多(duō)聚賴氨酸（poly-lysine）；科研應用上(shàng)，線性化PEI能(néng)與DNA或其他負電(diàn)荷生(shēng)物(wù)大分子簡單結合，正是這一(yī)特性，使得成為(wèi)一(yī)種非常行之有效的載體運輸介質（Vector carrier），即轉染試劑。 

Polyethylenimine, Linear, MW 25000（PEI 25K，PEI 25，000），是一(yī)種強大的，值得信賴且非常有效的瞬時轉染試劑。在HEK293和CHO表達系統，在各種生(shēng)産規模（從(cóng)96孔闆到(dào)100L生(shēng)物(wù)反應器(qì)），PEI都提供連續性的高(gāo)基因表達水(shuǐ)平。每年(nián)都有更多(duō)的研究者和公司使用Polysciences PEI在關鍵工(gōng)作中獲得優勢。與大多(duō)數其他選擇相(xiàng)比，使用PEI配制轉染試劑在整個(gè)轉染實驗降低(dī)多(duō)達40%的開(kāi)銷。

化學性質：

訂購信息：【原裝進口，現貨供應】 

使用方法【來源于：CSH protocols，僅作交流學習】：

1. Pour ~450 mL of Milli-Q H2O into a 500-mL glass beaker.

2. Add 500 mg of PEI to the beaker with stirring.

3. Add concentrated HCl drop-wise to the solution to pH< 2.0.

4. Stir until the PEI is dissolved (~2-3 h). Maintain the pH< 2.0 throughout.

Approximately 800 μL of 12 M HCl will be required for full PEI dissolution. There may still be some small fiber particles that will not dissolve.

5. Add concentrated NaOH drop-wise to the solution to pH 7.0.

Approximately 500 μL of 10 M NaOH will be required to neutralize the PEI solution.

6. Pour the solution into a 500-mL glass cylinder. Adjust the final volume to 500 mL with Milli-Q H2O.

7. Filter-sterilize the solution through a 0.22-μm membrane.

8. Store aliquots of the desired volume at -20°C.

應用文獻【基因轉染用途】：

Zhong Z, Feijen J, Lok MC, Hennink WE, Christensen LV, Yockman JW, Kim YH, Kim SW. (2005). Low molecular weight linear polyethylenimine-b-poly(ethylene glycol)-b-polyethylenimine triblock copolymers: synthesis, characterization, and in vitro gene transfer properties.Biomacromolecules. 6(6):3440-8.

Sharon E. Reed, Elizabeth M. Staley, John P. Mayginnes, David J. Pintel(2006). Transfection of mammalian cells using linear polyethylenimine is a simple and effective means of producing recombinant adeno-associated virus vectors.Journal of Virological Methods.138(1–2): 85–98.

Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy（2013）Transient Mammalian Cell Transfection with Polyethylenimine (PEI).Methods Enzymol. 529: 227–240.

Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci U S A. 102(16):5679-84.

Durocher, Y., Perret, S. & Kamen, A. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic acids research 30, E9 (2002). doi:10.1093/nar/30.2.e9

Wulhfard, S., Baldi, L., Hacker, D. L. & Wurm, F. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of Biotechnology 148, 128–132 (2010). doi:10.1016/j.jbiotec.2010.05.003

Baranyi, L. et al. Rapid Generation of Stable Cell Lines Expressing High Levels of Erythropoietin, Factor VIII, and an Antihuman CD20 Antibody Using Lentiviral Vectors. Human Gene Therapy Methods 24, 214–227 (2013). doi:10.1089/hgtb.2013.002

Siegman S, Truong NF, Segura T. (2015). Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation. Acta Biomater. in Press, doi:10.1016/j.actbio.2015.09.020

——Written/Edited by V. Shallan【版權歸MKBio懋康所有】

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