Stemfactor™ Activin A, Human Recombinant 重組人激活素A

産品關鍵詞：

naïve stem cells 初始幹細胞；neural progenitor cells 神經祖細胞；WH-4-023；CHIR99021；Stemfactor™ LIF；

産品描述：

Stemfactor™ Activin A是一(yī)種結晶級别的重組蛋白(bái)制備物(wù)，提供非常高(gāo)的純度。激活素A（Activin A）具有雙重功能(néng)，不僅能(néng)維持幹細胞潛能(néng)也能(néng)促進分化。成熟的重組人激活素A是2條116個(gè)氨基酸殘基組成的βA亞基通(tōng)過二硫鍵連接的二聚體，分子量約25kDa。

Stemgent公司提供支持初始潛能(néng)幹細胞應用需要的5i/L/A培養基添加劑（5i/L/A media supplment）所有成分，如下(xià)：

産品特性

1. 來源：E.Coli

2. 配方：Lyophilized from 20% acetonitrile

3. 氨基酸序列：GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS

4. Uniprot序列号：P08476, residues 311-426

5. 純度：＞98%（SDS-PAGE）

6. 内毒素水(shuǐ)平：Less than 1.0 EU/μg of Activin A as determined by the LAL method.

7. 生(shēng)物(wù)活性：The ED50 is less than 6 ng/ml as determined by its ability to inhibit the proliferation of mouse MPC-11 cells.

8. 重溶：Centrifuge briefly and reconstitute Activin A in 10 mM HCl before dilution in cell culture medium. Avoid freeze-thaw cycles as it can result in loss of activity.

9. 保存和穩定性：Activin A is shipped at room temperature. Lyophilized Activin A is stable for up to 6 months from date of receipt when stored at -20°C to -80°C.  Reconstituted Activin A, at concentrations greater than 0.1 mg/ml, is stable for up to 3 months when stored at -20°C and for 1 year when stored at -80°C

訂購信息：原裝正品，歡迎訂購，聯系電(diàn)話：021-54736159，QQ：2971634497。

引用文獻：

• Villa-Diaz LG; Kim JK; Laperle A; Palecek SP; Kresbach PH. "Inhibition of focal adhesion kinase signaling by integrin a6b1 supports human stem cell self-renewal." Stem Cells doi: 10.1002/stem.2349 (2016)

• Zhu S; Russ HA; Wang X; Zhang M; Ma T; Xu T; Tang S; Hebrok M; Deng S. "Human pancreatic beta-like cells converted from fibroblasts." Nature Commun 7:10080 (2016)

• Saxena S; Ronn RE; Guibentif C; Moragheba R; Woods N-B. "Cyclic AMP signaling through Epac axis modulates human hemogenic endothelium and enhances hematopoeitic cell generation." Stem Cell Rep doi:10.1016/j.stemcr.2016.03.006 (2016)

• Mora-Castilla S; To C; Vaezeslami S; Morey R; Srinivasan S; Dumdie JN; Cook-Andersen H; Jenkins J; Laurent LC. "Miniaturization technologies for efficient single-cell library preparation for next-generation sequencing." J Lab Automation 2016:1 (2016)

• Qian X. "Defining the mechanism by which synthetic polymer surfaces support human pluripotent stem cell self-renewal." PhD Thesis, University of Michigan (2015)

• Villa-Diaz LG; Kim JK; Lahann J; Kresbach PH. "Derivation and long-term culture of transfene-free human induced pluripotent stem cells on synthetic substates." Stem Cells Trans Med 3:1410 (2014)

• Bukong, T.N., Lo, T., Szabo, G., and Dolganiuc, A. (2012) Novel developmental biology-based protocol of embryonic stem cell differentiation to morphologically sound and functional yet immature hepatocytes. Liver Int. doi: 10.1111/j.1478-3231.2011.02743.x. [Epub ahead of print]

• Kunisada, Y., Tsubooka-Yamazoe, N., Shoji, M., and Hosoya, M. (2012) Small molecules induce efficient differentiation into insulin-producing cells from human induced pluripotent stem cells. Stem Cell Res 8: 274-284.

• McCracken, K.W., Howell, J.C., Wells, J.M., and Spence, J.R. (2011) Generating human intestinal tissue from pluripotent stem cells in vitro. Nat Protoc 6: 1920-1928.

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• 訂購信息：

  電(diàn)話：021-54736159

  QQ：2971634497

  郵箱：sales@maokangbio.com

  上海夢澤生物科技有限公司

   

   

   — —Written/Edited by V. Shallan【版權歸MKBio懋康所有】

   

   

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