産品簡介:
Minimal
SD Base SD基礎培養基【限時促銷:2024.3.18~24.6.30】
産品标簽
YPD
Medium(YEPD Medium);YPDA
Medium;YNB;Minimal
SD Base;Dropout (DO) Supplements 省卻成分添加劑;
産品信息
産品描述
Minimal
SD Base,即Minimal Synthetical Defined Medium,是在無氨基酸酵母氮源基礎(YNB)上(shàng)添加單一(yī)碳源如葡萄糖、半乳糖、棉子糖和琥珀酸鹽或多(duō)碳源搭配而合成的培養基基礎。常常與省卻成分添加劑(Dropout (DO) Supplements)聯合使用,以确認酵母菌的營養缺陷表型和篩選酵母轉化子。
保存與運輸方法
保存:室溫幹燥保存,至少2年(nián)穩定。
運輸:室溫運輸。
培養基配制
-
加入相(xiàng)應量的Minimal SD Base和DO Supplements【見(jiàn)下(xià)表1】到(dào)1L去離子水(shuǐ),攪拌使其溶解。
-
重要:測試培養基pH是否為(wèi)5.8,若有必要用弱酸或弱堿調整pH。
-
121℃高(gāo)壓滅菌15min。
-
柔光(guāng)下(xià)4℃保存高(gāo)壓滅菌後的液體培養基。
注意事(shì)項
為(wèi)了您的安全和健康,請穿實驗服并戴一(yī)次性手套操作。
相(xiàng)關産品
貨号
|
名稱
|
規格
|
MS6301-350G
|
YPD Agar Medium YPD瓊脂培養基
|
250g
|
MS6302-250G
|
YPD Medium YPD液體培養基
|
250g
|
MS6303-350G
|
YPDA Agar Medium YPDA瓊脂培養基
|
350g
|
MS6304-250G
|
YPDA Medium YPDA液體培養基
|
250g
|
MS6305-100G
|
YNB Without Amino Acids酵母氮源基礎(無氨基酸)
|
100g
|
MS6305-500G
|
YNB Without Amino Acids酵母氮源基礎(無氨基酸)
|
500g
|
MS6311-467G
|
Minimal SD Agar Base SD瓊脂基礎培養基
|
467g
|
MS6312-267G
|
Minimal SD Base SD基礎培養基
|
267g
|
MS6313-270G
|
Minimal SD Agar Base Gal/Raf SD瓊脂基礎培養基(含半乳糖/棉子糖)
|
270g
|
MS6314-185G
|
Minimal SD Base Gal/Raf SD基礎培養基(含半乳糖/棉子糖)
|
185g
|
表1 酵母培養基配制濃度表
培養基類型
|
名稱
|
貨号
|
終濃度(g/L)
|
Rich Media
富集培養基
|
YPD Agar Medium
|
MS6301-350G
|
70
|
YPD Medium
|
MS6302-250G
|
50
|
YPDA Agar Medium
|
MS6303-350G
|
70
|
YPDA Medium
|
MS6304-250G
|
50
|
Minimal Media
基本培養基
|
Minimal SD Agar Base
|
MS6311-467G
|
46.7
|
Minimal SD Base
|
MS6312-267G
|
26.7
|
Minimal SD Agar Base Gal/Raf
|
MS6313-270G
|
54
|
Minimal SD Base Gal/Raf
|
MS6314-185G
|
37
|
Single Dropout Supplements (SDO)
單一(yī)省卻成分添加劑
|
DO Supplement -His
|
MS6316-10G
|
1.29
|
DO Supplement -Leu
|
MS6317-10G
|
1.29
|
DO Supplement -Trp
|
MS6318-10G
|
1.29
|
DO Supplement -Ura
|
MS6319-10G
|
1.29
|
Double Dropout Supplements (DDO)
雙省卻成分添加劑
|
DO Supplement -His/-Leu
|
MS6320-10G
|
1.19
|
DO Supplement -His/-Ura
|
MS6321-10G
|
1.27
|
DO Supplement -Leu/-Trp
|
MS6322-10G
|
1.19
|
DO Supplement -Met/-Trp
|
MS6323-10G
|
1.27
|
DO Supplement -Trp/-Ura
|
MS6324-10G
|
1.27
|
DO Supplement -Trp/-His
|
MS6325-10G
|
1.27
|
DO Supplement -Ura/-Leu
|
MS6326-10G
|
1.27
|
Triple Dropout Supplements (TDO)
三省卻成分添加劑
|
DO Supplement -His/-Leu/-Trp
|
MS6331-10G
|
1.17
|
DO Supplement -His/-Leu/-Ura
|
MS6332-10G
|
1.17
|
DO Supplement -His/-Trp/-Ura
|
MS6333-10G
|
1.25
|
DO Supplement -Leu/-Met/-Trp
|
MS6334-10G
|
1.17
|
DO Supplement -Leu/-Trp/-Ura
|
MS6335-10G
|
1.17
|
Quadruple Dropout Supplements (QDO)
四省卻成分添加劑
|
DO Supplement -Ade/-His/-Leu/-Trp
|
MS6341-10G
|
1.15
|
DO Supplement -His/-Leu/-Met/-Trp
|
MS6342-10G
|
1.15
|
DO Supplement -His/-Leu/-Trp/-Ura
|
MS6343-10G
|
1.15
|
— — Written/Edited by V. Shallan【版權歸MKBio懋康所有】
上海夢澤生物科技有限公司是一(yī)家涉足于生(shēng)命科學和生(shēng)物(wù)技(jì)術(shù)領域研究的試劑、儀器(qì)和實驗室消耗品與實驗服務工(gōng)作,主要從(cóng)事(shì)細胞生(shēng)物(wù)學、植物(wù)學、分子生(shēng)物(wù)學、免疫學、生(shēng)物(wù)化學、蛋白(bái)組學。生(shēng)物(wù)制藥與診斷試劑研發生(shēng)産等領域。 本公司秉承“以人為(wèi)本,以誠為(wèi)信、合同守信”的經營理念。堅持"品質保障"的原則為(wèi)廣大客戶提供優質産品。
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