目錄号 | PK201 | 售價 | 7176.00元 | ||||||||||||||||||||||||||||||||||||||||||||||||
規格 | For 35 sections | 運輸溫度 | 室溫 | ||||||||||||||||||||||||||||||||||||||||||||||||
其他名稱 | FD NeuroApop™ Kit | 保存溫度 | 室溫 | ||||||||||||||||||||||||||||||||||||||||||||||||
CAS号 | N/A | 有效期 | |||||||||||||||||||||||||||||||||||||||||||||||||
應用 | 神經元凋亡染色 | 訂購數量 |
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産品簡介: FD NeuroApop™ Kit FD神經元凋亡檢測試劑盒
搜索關鍵詞: FD NeuroTechnologies, Inc.;Golgi-Cox impregnation 浸染法;Neuronal apoptosis 神經元凋亡;Fluoro-Jade B;In situ DNA nick-end labeling (TUNEL) 原位缺口末端标記法;
産品描述: FD NeuroTechnologies, Inc(USA)公司生(shēng)産的FD神經元凋亡檢測試劑盒(FD NeuroApop™ Kit)特别研發用于中樞神經系統組織切片中的神經元凋亡檢測,基于原位DNA缺口末端标記技(jì)術(shù)(TUNEL法)。該技(jì)術(shù)使用末端脫氧核糖核酸轉移酶(TdT)催化生(shēng)物(wù)素偶聯的脫氧尿嘧啶插入DNA片段自(zì)由的3’-OH末端,其被認為(wèi)是凋亡最典型特征之一(yī)。之後插入DNA的生(shēng)物(wù)素使用親和素-生(shēng)物(wù)素複合物(wù)(ABC)方法得以信号擴大,并在光(guāng)學顯微鏡下(xià)進行可視化鑒定。
FD神經元凋亡檢測試劑盒(FD NeuroApop™ Kit)提供的試劑和步驟都經過優化以能(néng)在特異性和靈敏度上(shàng)高(gāo)效檢測凋亡神經元,且維持極低(dī)背景。此試劑盒适用于冰凍和石蠟切片,以及培養細胞。本試劑盒整個(gè)操作流程約需4h。
試劑盒組分(PK201):
應用實例:
圖1. Detection of apoptotic neurons with FD NeuroApop™ Kit. Paraffin section (10µm) cut from a dorsal ganglion of a mouse embryo (E17). The section was processed with FD NeuroApop™ kit and counterstained with methyl green. Sections courtesy of Drs. Michael Vogel and Lisa Qiu.
訂購信息:原裝正品,歡迎來電(diàn)咨詢。電(diàn)話:021-54736159,18121428569。QQ:3308240863,2971634497。網址:www.maokangbio.com
幾十篇使用文獻的數據支持,是值得信賴的科研輔助精品。詳見(jiàn)操作手冊。 PK201-FD NeuroApop™ Kit User Mannual PK101-FD Apop™ Kit User Manual
技(jì)術(shù)文獻: [1] Gavrieli Y, Sherman Y, and Ben-Sasson SA. (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol. 119:493-501.
[2] Wyllie AH. (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature. 284:555-6.
[3] Arends MJ, Morris RG, and Wyllie AH. (1990) Apoptosis: the role of the endonuclease. Amer. J. Pathol 136:593-608.
[4] Hsu SM, Raine L, and Fanger H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29:577-80.
— —Written/Edited by V. Shallan【版權歸MKBio懋康所有】
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