目錄号 | 04942078001-100MG | 售價 | 650.00元 |
規格 | 100MG | 運輸溫度 | 4℃ |
其他名稱 | 中性蛋白(bái)酶,分散酶 | 保存溫度 | |
CAS号 | N/A | 有效期 | |
應用 | 訂購數量 |
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産品簡介: Dispase® II (neutral protease, grade II) 産品信息: 産品名稱 :中性蛋白(bái)酶/分散酶/Neutral protease/Dispase 英文名稱: Neutral protease;Dispase 産品性狀:黃褐色粉末,是枯草(cǎo)杆菌經深層發酵培養後産生(shēng)的代謝物(wù),經鹽析、幹燥、磨細成粉制成,易溶于水(shuǐ)。用微生(shēng)物(wù)生(shēng)産的中性蛋白(bái)酶,大多(duō)數是金屬酶,一(yī)分子蛋白(bái)酶中含一(yī)原子鋅,分子量35000~40000,等電(diàn)點8~9,是微生(shēng)物(wù)蛋白(bái)酶中最不穩定的酶,易自(zì)溶,會(huì)造成分子量的明顯減少 CAS号:9068-59-1 級别:BR 活力:≥50units/mg 活力定義:1g固體酶粉,在30 oC,PH7.5條件(jiàn)下(xià),1分鍾水(shuǐ)解酪素産生(shēng)1ug酪氨酸為(wèi)1個(gè)酶活力單位,以u/g表示 穩定pH:5.5~8.5 最适PH:6.8~7.0 最适溫度:45~50℃ 用途:生(shēng)化研究,提取多(duō)糖和水(shuǐ)解各類植物(wù)蛋白(bái)。中性條件(jiàn)下(xià),催化分解蛋白(bái)質或高(gāo)分子肽鍵,其産物(wù)為(wèi)小(xiǎo)分子的蛋白(bái)胨、小(xiǎo)肽以及個(gè)别的氨基酸 儲存條件(jiàn) :2~8℃ Roche相(xiàng)關資料如下(xià) EC 3.4.24.4 One unit is the enzyme activity which liberates Folin-positive amino acids and peptides corresponding to 1 µmol tyrosine in 1 minute under assay conditions. A practical comparison of Roche units of Dispase® II with those cited in the Japanese literature (where dispase concentrations of 1,000 to 2,000 units/ml are not uncommon) suggests that one Roche unit equals approximately 600 Japanese units of dispase. Enzyme activity: ≥0.8 U/mg (+37°C, casein as substrate, pH 7.5) Inhibitors: EDTA, EGTA, Hg2+, other heavy metals. Dispase is not inhibited by serum. Specificity: Dispase is a nonspecific metalloprotease. Working concentration: 0.6 to 2.4 U/ml |