産品簡介:
MPP+ iodide (N-Methyl-4-Phenylpyridinium Iodide)
産品标簽
MPP+ iodide;MPTP;帕金森病動物(wù)模型;Ferroptosisinhibitor;Necrostatin-1;Ferrostatin-1 (Fer-1);CAS:36913-39-0;
産品信息:
産品名稱
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産品編号
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CAS NO.
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規格
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價格(元)
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MPP+ iodide (N-Methyl-4-Phenylpyridinium Iodide)
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MZ6402-50MG
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36913-39-0
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50mg
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922
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MPP+iodide (N-Methyl-4-Phenylpyridinium Iodide)
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MZ6402-100MG
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36913-39-0
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100mg
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1592
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MPP+iodide (N-Methyl-4-Phenylpyridinium Iodide)
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MZ6402-250MG
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36913-39-0
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250mg
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3522
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産品描述
MPP+iodide (N-Methyl-4-Phenylpyridinium Iodide)是神經毒素MPTP的活性代謝物(wù),在帕金森病動物(wù)模型中選擇性破壞多(duō)巴胺能(néng)神經元。MPP+主要通(tōng)過抑制線粒體電(diàn)子傳遞鏈複合物(wù)I,引起ATP耗竭和氧化應激增高(gāo),從(cóng)而誘導神經毒性。MPP+誘導Necrostatin-1 (Nec-1)和 Ferrostatin-1 (Fer-1)敏感的SH-SY5Y細胞壞死,但MPP+誘導的壞死性細胞死亡與鐵壞死是不同的生(shēng)理過程。
産品特性
1) 化學名:1-Methyl-4-phenylpyridin-1-ium iodide
2) 同義名:N-Methyl-4-Phenylpyridinium Iodide; 1-methyl-4-phenyl-pyridinium, monoiodide; MPP+;N-甲基-4-苯基吡啶鎓碘化物(wù);1-甲基-4-苯基-吡啶鎓碘化物(wù);1-甲基-4-苯基吡啶碘化物(wù);
3) CAS NO:36913-39-0
4) 分子式:C12H12IN
5) 分子量:297.1
6) 純度:≥98%
7) 外觀:固體
8) 溶解性:溶于H2O(100mM)
9) 化學結構式:
保存與運輸方法
保存:-20ºC避光(guāng)幹燥保存,至少2年(nián)有效。
運輸:冰袋運輸。
注意事(shì)項
1) 關于化合物(wù)溶解性:産品特性内的“≥”表明溶于标示濃度,但飽和溶解度未知。不同批次化合物(wù)的溶解度會(huì)有差異。
2) 為(wèi)了讓化合物(wù)更好的溶解,可通(tōng)過37℃加熱或(和)超聲波水(shuǐ)浴中震動片刻來處理。若實驗所需濃度過大甚至達産品溶解極限,請添加助溶劑助溶或自(zì)行調整濃度。
3) 本品僅用作科研用途,不可用于臨床或診療用途。
4) 為(wèi)了您的安全和健康,請穿實驗服并戴一(yī)次性手套操作。
儲存液制備
質量
溶劑體積
濃度
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1mg
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5mg
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10mg
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1mM
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3.3654 mL
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16.8271 mL
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33.6542mL
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5mM
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0.6731mL
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3.3654 mL
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6.7308mL
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10mM
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0.3365 mL
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1.6827 mL
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3.3654 mL
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【溫馨提示】:請根據産品在不同溶劑中的溶解度選擇合适的溶劑配制儲備液;MPP+在溶液中不穩定,建議新鮮配制溶液且立即使用。配制好的溶液需避光(guāng)保存和使用。
使用方法【源自(zì)文獻,僅作參考】
文獻1,Stahl K, Rahmani S, Prydz A, Skauli N, MacAulay N, Mylonakou MN, Torp R, Skare Ø, Berg T, Leergaard TB, Paulsen RE, Ottersen OP, Amiry-Moghaddam M. Targeted deletion of the aquaglyceroporin AQP9 is protective in a mouse model of Parkinson's disease. PLoS One. 2018 Mar 22;13(3):e0194896. doi: 10.1371/journal.pone.0194896. PMID: 29566083; PMCID: PMC5864064.
體内研究(動物(wù)模型):
動物(wù)模型(Animal Model):C57BL/6J WT and Aqp9-/- mice
配制方法(Formulation):MPP+ (7.5 μg dissolved in saline)
實驗方法(Assay):C57BL/6J WT (n = 34) and Aqp9-/- mice (n = 29) were deeply anesthetized with zoletil mixture, Rompunand Fentanyl and then subjected tostereotaxic intrastriatal injections of MPP+ (7.5 μg dissolved in saline) or saline. 1 μl MPP+ solution or saline was injected into the striatum 0.6 mm anterior to Bregma, 2.2 mm laterally and 3.2 mm ventrally at 12 μl/hr using a syringe pump. MPP+ was protected against light during the procedure. The injector was left for 5 minutes to allow diffusion before suture.
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文獻2,Xu J, Gao X, Yang C, Chen L, Chen Z. Resolvin D1 Attenuates Mpp+-Induced Parkinson Disease via Inhibiting Inflammation in PC12 Cells. Med Sci Monit. 2017 Jun 2;23:2684-2691. doi: 10.12659/msm.901995. PMID: 28572562; PMCID: PMC5465971.
體外研究(細胞實驗):
細胞類型(Cell types):PC12 cells
實驗方法(Assay):The in vitro PD model was established by treatment of PC12 cells with250 μM MPP+ for approximately 24 hours.Different concentrations of RvD1 (50, 100, 200 nM) were administrated two hours prior to MPP+ treatment, afterwards, PC12 cells were used for following experiments.
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文獻3,Christensen C, Þorsteinsson H, Maier VH, Karlsson KÆ. Multi-parameter Behavioral Phenotyping of the MPP+ Model of Parkinson's Disease in Zebrafish. Front Behav Neurosci. 2020 Dec 18;14:623924. doi: 10.3389/fnbeh.2020.623924. PMID: 33390914; PMCID: PMC7775599.
體内研究(動物(wù)模型):
動物(wù)模型(Animal Model):Zebrafish larvae
配制方法(Formulation):Both MPP+ and PBA were dissolved in the system water.
實驗方法(Assay):Zebrafish larvae were incubated between 1 and 5 dpf in the presence or absence of 500 μM MPP+ at a total volume of 25 ml system water. The drug solutions were freshly prepared and replaced between 11:00 and 12:00 am at 2, 3, and 4 dpf.Deceased larvae were removed daily, but no differences in mortality were observed between MPP+ exposed larvae and untreated larvae.
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— —Written/Edited by V. Shallan【版權歸MKBio懋康所有】
上海夢澤生物科技有限公司是一(yī)家涉足于生(shēng)命科學和生(shēng)物(wù)技(jì)術(shù)領域研究的試劑、儀器(qì)和實驗室消耗品與實驗服務工(gōng)作,主要從(cóng)事(shì)細胞生(shēng)物(wù)學、植物(wù)學、分子生(shēng)物(wù)學、免疫學、生(shēng)物(wù)化學、蛋白(bái)組學。生(shēng)物(wù)制藥與診斷試劑研發生(shēng)産等領域。 本公司秉承“以人為(wèi)本,以誠為(wèi)信、合同守信”的經營理念。堅持"品質保障"的原則為(wèi)廣大客戶提供優質産品。
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