質量

溶劑體積

濃度

25µg

500µg

1mg

1mM

40.1394 µL

0.8027 mL

1.6056 mL

5mM

8.0279 µL

0.1605 mL

0.3211 mL

文獻1，Yasuo Hayashi, et al.(2006) Effects of bafilomycin A1, a vacuolar type H+ ATPase inhibitor, on the thermosensitivity of a human pancreatic cancer cell line, International Journal of Hyperthermia, 22:4, 275-285

體外研究（細胞水(shuǐ)平）：

細胞類型（Cell Type）：AsPC-1 cells

配制方法（Formulation）：Bafilomycin A1 was dissolved in DMSO and then diluted to appropriate concentrations with the culture medium. The final concentration of DMSO in the medium was 1% or less.

實驗方法（Assay）：The thermosensitizing effect of bafilomycin A1 and EIPA on AsPC-1 cells was quantified using a MTT colorimetric assay. 100μl cell suspension (1×10⁵ cells/ml) was added to each well of a 96-well plate and incubated for 72 h at 37℃. The culture medium was then removed and 100 ml of pH 6.8 or 7.4 HEPES- buffered RPMI 1640 medium containing 1 mM bafilomycin A1 and/or 10 mM EIPA were added to each well. The plates were capped tightly and immersed in a 37C or 44C water bath for varying lengths of time. MTT assay was done to detect cell viability.

體内研究（動物(wù)模型）：

動物(wù)模型（Animal Model）：BALB/cA nude mice (Jcl-nu)

配制方法（Formulation）：For in vivo studies, Bafilomycin A1 was dissolved in ethanol and then diluted to appropriate concentrations with PBS. The final concentration of ethanol in the medium was 1% or less.

實驗方法（Assay）：AsPC-1 cells were injected s.c. into the right thigh of 6-week-old male mice. When tumors grew to ~150 mm³, they were divided randomly into eight experimental groups: a control group, a bafilomycin A1 (1.0 mg kg^-1) treated group, a EIPA (3.0 mg kg^-1 ) treated group, a bafilomycin A1 (1.0 mg kg^-1) and EIPA (3.0 mg kg^-1) treated group, a heated-only group, a heated group treated with bafilomycin A1 (1.0 mg kg^-1), a heated group treated with EIPA (3.0 mg kg^-1), a heated group treated with bafilomycin A1 (1.0 mg kg^-1) and EIPA (3.0 mg kg^-1). The non-heated groups received i.p. injections of 1.0mg kg^-1 of bafilomycin A1 and/or 3.0 mg kg^-1 of EIPA or PBS. The heated groups were injected IP with the drugs and tumours were heated beginning 60 min.