BMPO (Spin trapping reagent)自(zì)旋捕獲劑

産品标簽

BMPO；DEPMPO；EMPO；Spin trapping reagent自(zì)旋捕獲劑；電(diàn)子順磁共振波譜（EPR）；CAS: 387334-31-8；

産品信息

産品描述

BMPO（5-tert-butoxycarbonyl 5-methyl-1-pyrroline N-oxide）是一(yī)種新型高(gāo)效且高(gāo)穩定型的硝酮自(zì)旋捕獲劑（Nitrone Spin Trapping Reagent），由美國(guó)威斯康星醫(yī)學院Kalyanaraman教授的實驗室團隊開(kāi)發，非常适合用于特異性檢測和鑒定體内外形成的硫自(zì)由基、羟基自(zì)由基（•OH）和超氧陰離子自(zì)由基（•O2-），通(tōng)過形成用電(diàn)子順磁共振波譜（EPR）可區分的加合物(wù)來測定[1-2]。

BMPO具有以下(xià)特點：

①特異性高(gāo)，半衰期長(cháng)：其它的硝酮自(zì)旋捕獲劑比如：DMPO，難以輕易區分超氧陰離子和羟基自(zì)由基，因産生(shēng)的DMPO-超氧加合物(wù)（半衰期t½=45s）瞬間衰減産生(shēng)DMPO-羟基加合物(wù)。與最近開(kāi)發的自(zì)旋捕獲劑：DEPMPO和EMPO類似，BMPO-超氧加合物(wù)不會(huì)衰減生(shēng)成羟基加合物(wù)，但是，BMPO-超氧加合物(wù)的半衰期最長(cháng)（t½=23min）。

②産品穩定性高(gāo)：DEPMPO和EMPO是液體自(zì)旋捕獲劑，通(tōng)常被硝基氧雜(zá)質污染，且效期有限。BMPO是固體的環形硝酮，通(tōng)過結晶以高(gāo)度純化的狀态提供，能(néng)夠保存更長(cháng)的周期，且不用擔心降解。

③更高(gāo)的信噪比：BMPO衍生(shēng)的加合物(wù)在各自(zì)的EPR光(guāng)譜中具有更高(gāo)的信噪比，使其更适合用于檢測細胞懸浮液中的亞硫酸、羟基和甲基自(zì)由基。

④高(gāo)水(shuǐ)溶性：水(shuǐ)溶性好，更利于水(shuǐ)相(xiàng)體系自(zì)由基的研究，尤其是生(shēng)物(wù)體系的自(zì)由基研究。

産品特性

1） CAS NO：387334-31-8

2） 化學名：3,4-dihydro-2-methyl-1,1-dimethylethyl ester-2H-pyrrole-2-carboxylic acid-1-oxide

3） 同義名：5-tert-butoxycarbonyl 5-methyl-1-pyrroline N-oxide; BocMPO;

4） 分子式：C10H17NO3

5） 分子量：199.2

6） 純度：≥98%

7） 外觀：結晶或結晶性粉末

8） 溶解性：溶于水(shuǐ)、PBS（pH 7.2, 10mg/ml）、DMSO（25mg/ml）、無水(shuǐ)乙醇（25mg/ml）

8）化學結構式：

保存與運輸方法

保存：-20ºC幹燥保存，2年(nián)有效。 

運輸：冰袋運輸。

注意事(shì)項

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參考文獻

1. Zhao, H., Joseph, J., Zhang, H., et al. Synthesis and biochemical applications of a solid cyclic nitrone spin trap: A relatively superior trap for detecting superoxide anions and glutathiyl radicals. Free Radic. Biol. Med. 31(5), 599-606 (2001).

2. Khan, N., Wilmont, C.M., Rosen, G.M., et al. Spin traps: In vitro toxicity and stability of radical adducts. Free Radical Biology & Medicine 34(11), 1473-1481 (2003).

應用示例（僅作參考）

1.文獻來源：Mitchell DG, Rosen GM, Tseitlin M, Symmes B, Eaton SS, Eaton GR. Use of rapid-scan EPR to improve detection sensitivity for spin-trapped radicals. Biophys J. 2013 Jul 16;105(2):338-42. doi: 10.1016/j.bpj.2013.06.005. PMID: 23870255; PMCID: PMC3714875. Editedby MKBIO

①黃嘌呤-黃嘌呤氧化酶體系内超氧陰離子（O2⋅−）生(shēng)成的測定：Typically, xanthine oxidase (0.04 U/mL) was added to pH ∼7.4 sodium phosphate buffer (50 mM) containing DTPA (1 mM) and hypoxanthine (0.5–400 μM, final concentration) to achieve rates of O2⋅− formation that ranged from 0.1 to 6.0 μM/min. We estimated the superoxide formation rate by monitoring the SOD-inhibitable reduction of ferricytochrome c (80 μM) at room temperature. Spin trapping was performed by addition of 100 mM BMPO in pH ∼7.4 phosphate-buffered saline (PBS; 50 mM) containing 1 mM DTPA to the solution of hypoxanthine and xanthine oxidase to achieve a final BMPO concentration of 50 mM in the reaction mixture. EPR spectra were recorded 10 min after mixing reagents. The half-life of BMPO-OOH at ambient temperature is reported to be ∼23 min. Solutions for control experiments contained SOD (30 U/mL).

Figure 2 Comparison of CW and rapid-scan spectra of BMPO-OOH in solution with a O2⋅− production rate of 0.1μM/min, recorded 10 min after mixing reagents. The O2⋅− was produced by a hypoxanthine/xanthine oxidase mixture. The concentration of BMPO-OOH is ∼0.3μM. (A) CW spectrum obtained with 55 G sweep width, 0.75 G modulation amplitude, single 30 s scan, 15 ms conversion time, 10 ms time constant, and 20 mW (B1 = 170 mG) microwave power. (B) Deconvolved rapid-scan spectrum obtained with 55 G scan width, 51 kHz scan frequency, and 53 mW (B1 = 250 mG) microwave power. Segments consisting of 12 sinusoidal cycles were averaged 100 k times, with a total data acquisition time of ∼30 s.

2.文獻來源：Wang, Z., Zhang, Y., Ju, E.et al. Biomimetic nanoflowers by self-assembly of nanozymes to induce intracellular oxidative damage against hypoxic tumors.Nat Commun 9,3334 (2018). https://doi.org/10.1038/s41467-018-05798-x 

①缺氧下(xià)的ESR測定（O2•−，•OH）：For O2•− detection, the pre-deoxidized PBS (pH 5.0, 25 mM) contained 25 mM BMPO, 20 μg mL−1 MnO2@PtCo nanoflowers, 100 μM H2O2, 50% DMSO was prepared. After incubation of 5 min, ESR spectra were recorded. For •OH detection, the pre-deoxidized PBS (pH 5.0, 25 mM) contained 25 mM BMPO, 20 μg mL−1 MnO2@PtCo nanoflowers, 100 μM H2O2, 0.25 U mL−1 SOD was prepared. After incubation of 5 min, ESR spectra were recorded. The following instrument settings were used for collecting ESR spectra: 1 G field modulation, 100 G scan range, and 20 mW microwave power.

Fig f ESR spectra of BMPO/•OOH adducts from different groups in the hypoxic H2O2 (100 μM) condition upon addition of DMSO.g ESR spectra of BMPO/•OH adducts were collected from different samples in the hypoxic H2O2 (100 μM) condition upon addition of SOD. Data were presented as mean ± s.d. (n= 5).

相(xiàng)關産品

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